Scanometric nanomolar lead (II) detection using DNA-functionalized gold nanoparticles and silver stain enhancement

被引：32

作者：

Chen, Beibei ^{[1
]}

Wang, Zebo ^{[1
]}

Hu, Dongxue ^{[1
]}

Ma, Qingqing ^{[1
]}

Huang, Linna ^{[1
]}

Xv, Chengyin ^{[2
]}

Guo, Zhiyong ^{[1
]}

Jiang, Xiaohua ^{[3
]}

机构：

[1] Ningbo Univ, Fac Mat Sci & Chem Engn, State Key Lab Base Novel Funct Mat & Preparat Sci, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Univ, Coll Sci & Technol, Ningbo 315212, Zhejiang, Peoples R China

[3] Shenzhen Polytech, Sch Appl Chem & Biol Technol, Shenzhen 518055, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 200卷

基金：

中国国家自然科学基金;

关键词：

Gold label silver stain (GLSS); DNAzyme; Gold nanoparticles; Lead (Pb2+) detection; FREE COLORIMETRIC DETECTION; DNAZYME; IONS; CATALYSIS;

D O I：

10.1016/j.snb.2014.04.066

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on gold label silver stain method, a facile, sensitive and selective scanometric method for the detection of lead (II) was developed. The main components of the method include "8-17" DNAzyme functionalized gold nanoparticles (AuNPi), capture DNA functionalized gold nanoparticles (AuNP2), and silver staining. In presence of lead (II), AuNPi was cleaved into different pieces that were captured by AuNP2; and became aggregated and formed network structures. These aggregate structures hindered the exposure of gold particles during the silver staining, resulting in a reduced gray values compared with that in the absence of lead (II). Using the gray value of silver stain signals, the developed scanometric assay realized a semi-quantitative detection of nanomolar levels of lead (II), ranging from 2 to 1000 nmol/L (about 0.4-200 ppb), without significant interference from other metal ions. Results suggested that this scanometric method is a simple, sensitive, selective, and portable tool for the onsite detection of nanomolar levels of lead (II). (C) 2014 Elsevier BM. All rights reserved.

引用

页码：310 / 316

页数：7