Isolation and Characterization of Collagen and Antioxidant Collagen Peptides from Scales of Croceine Croaker (Pseudosciaena crocea)

被引:132
|
作者
Wang, Bin [1 ]
Wang, Yu-Mei [1 ]
Chi, Chang-Feng [2 ]
Luo, Hong-Yu [1 ]
Deng, Shang-Gui [1 ]
Ma, Jian-Yin [1 ]
机构
[1] Zhejiang Ocean Univ, Sch Food & Pharm, Zhoushan 316022, Peoples R China
[2] Zhejiang Ocean Univ, Sch Marine Sci & Technol, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Peoples R China
来源
MARINE DRUGS | 2013年 / 11卷 / 11期
基金
中国国家自然科学基金;
关键词
croceine croaker (Pseudosciaena crocea); scale; acid soluble collagen (ASC); peptide; antioxidant activity; RADICAL-SCAVENGING ACTIVITIES; PEPSIN-SOLUBILIZED COLLAGEN; ACID-SOLUBLE COLLAGEN; FUNCTIONAL-PROPERTIES; PROTEIN HYDROLYSATE; GELATIN HYDROLYSATE; SPHYRNA-LEWINI; SKIN GELATIN; I COLLAGEN; PURIFICATION;
D O I
10.3390/md11114641
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Acid soluble collagen (ASC) from scales of croceine croaker (ASC-C) was successfully isolated with the yield of 0.37% +/- 0.08% (dry weight basis), and characterized as type I collagen on the basis of amino acid analysis and electrophoretic pattern. The antioxidant hydrolysate of ASC-C (ACH) was prepared through a two-stage in vitro digestion (4-h trypsin followed by 4-h pepsin), and three antioxidant peptides (ACH-P1, ACH-P2, and ACH-P3) were further isolated from ACH using ultrafiltration, gel chromatography, and RP-HPLC, and their amino acid sequences were identified as GFRGTIGLVG (ACH-P1), GPAGPAG (ACH-P2), and GFPSG (ACH-P3). ACH-P1, ACH-P2, and ACH-P3 showed good scavenging activities on hydroxyl radical (IC50 0.293, 0.240, and 0.107 mg/mL, respectively), DPPH radical (IC50 1.271, 0.675, and 0.283 mg/mL, respectively), superoxide radical (IC50 0.463, 0.099, and 0.151 mg/mL, respectively), and ABTS radical (IC50 0.421, 0.309, and 0.210 mg/mL, respectively). ACH-P3 was also effectively against lipid peroxidation in the model system. The antioxidant activities of three collagen peptides were due to the presence of hydrophobic amino acid residues within the peptide sequences. The collagen peptides might be used as antioxidant for the therapy of diseases associated with oxidative stress, or reducing oxidative changes during storage.
引用
收藏
页码:4641 / 4661
页数:21
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