Genome changes in the Toxoplasma gondii strains during laboratory passages in mice

被引:2
作者
Literák, I
Rychlík, I
机构
[1] Univ Vet & Pharmaceut Sci, Fac Vet Med, Dept Infect Dis & Vet Epidemiol, Brno 61242, Czech Republic
[2] Vet Res Inst, CS-62132 Brno, Czech Republic
关键词
Toxoplasma gondii; PCR/RFLP; RFLP/DNA; DNA fingerprinting; strains; pathogenicity; virulence;
D O I
10.2754/avb199968030203
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Three strains of Toxoplasma gondii isolated from rabbits (K6 and K9) and a cat (K24) in the Czech Republic in 1994-95 were maintained in a tissue cyst form in laboratory mice by injections of brain homogenate every 4 - 6 months. These strains were passaged 12 to 13 times before tachyzoites, from which DNA was isolated, were obtained. PCR/RFLP (restriction fragment length polymorphism) (ROP1 gene/DdeI restriction endonuclease) and RFLP/DNA (RFLP with chromosomal DNA) (TGR1E probe/PstI and San, restriction endonucleases) were used to characterize their genotypes. In the course of subsequent tissue cyst-tissue cyst passages, the pathogenicity of the strains increased and intraperitoneal passaging of tachyzoites from the peritoneal exudate two times weekly had to be used to facilitate the continuous maintenance. After the pathogenicity increase, the DNA was isolated from the tachyzoites after 6 to 48 tachyzoite-tachyzoite passages. Genotype differences were observed in the strain before and after increasing their pathogenicity. Both PCR/RFLP and RRLP/DNA patterns of the strain before and after increasing their pathogenicity were similar, and even identical, with previously described genotype characteristics of avirulent and virulent strains of T. gondii.
引用
收藏
页码:203 / +
页数:7
相关论文
共 19 条
[1]   Microsatellite in the beta-tubulin gene of Toxoplasma gondii as a new genetic marker for use in direct screening of amniotic fluids [J].
Costa, JM ;
Darde, ML ;
Assouline, B ;
Vidaud, M ;
Bretagne, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (10) :2542-2545
[2]   A FAMILY OF REPEATED DNA-SEQUENCES IN TOXOPLASMA-GONDII - CLONING, SEQUENCE-ANALYSIS, AND USE IN STRAIN CHARACTERIZATION [J].
CRISTINA, N ;
LIAUD, MF ;
SANTORO, F ;
OURY, B ;
AMBROISETHOMAS, P .
EXPERIMENTAL PARASITOLOGY, 1991, 73 (01) :73-81
[3]   A DNA-FINGERPRINTING METHOD FOR INDIVIDUAL CHARACTERIZATION OF TOXOPLASMA-GONDII STRAINS - COMBINATION WITH ISOENZYMATIC CHARACTERS FOR DETERMINATION OF LINKAGE GROUPS [J].
CRISTINA, N ;
DARDE, ML ;
BOUDIN, C ;
TAVERNIER, G ;
PESTREALEXANDRE, M ;
AMBROISETHOMAS, P .
PARASITOLOGY RESEARCH, 1995, 81 (01) :32-37
[4]   RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS AMONG TOXOPLASMA-GONDII STRAINS [J].
CRISTINA, N ;
OURY, B ;
AMBROISETHOMAS, P ;
SANTORO, F .
PARASITOLOGY RESEARCH, 1991, 77 (03) :266-268
[5]   ISOENZYME ANALYSIS OF 35 TOXOPLASMA-GONDII ISOLATES AND THE BIOLOGICAL AND EPIDEMIOLOGIC IMPLICATIONS [J].
DARDE, ML ;
BOUTEILLE, B ;
PESTREALEXANDRE, M .
JOURNAL OF PARASITOLOGY, 1992, 78 (05) :786-794
[7]  
Dubey J. P., 1988, TOXOPLASMOSIS ANIMAL
[8]  
Frenkel JK, 1997, PARASITOL RES, V83, P1, DOI 10.1007/s004360050197
[9]   LOSS OF STAGES AFTER CONTINUOUS PASSAGE OF TOXOPLASMA-GONDII AND BESNOITIA-JELLISONI [J].
FRENKEL, JK ;
DUBEY, JP ;
HOFF, RL .
JOURNAL OF PROTOZOOLOGY, 1976, 23 (03) :421-424
[10]   TOXOPLASMA-GONDII COMPRISES 3 CLONAL LINEAGES - CORRELATION OF PARASITE GENOTYPE WITH HUMAN-DISEASE [J].
HOWE, DK ;
SIBLEY, LD .
JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (06) :1561-1566