Simultaneous analysis of dopamine and homovanillic acid by high-performance liquid chromatography with wall-jet/thin-layer electrochemical detection

被引：54

作者：

Zhou, Yaping ^{[1
]}

Yan, Hongling ^{[1
]}

Xie, Qingji ^{[1
]}

Huang, Siyu ^{[1
]}

Liu, Jiali ^{[1
]}

Li, Zou ^{[1
]}

Ma, Ming ^{[1
]}

Yao, Shouzhuo ^{[1
,2
]}

机构：

[1] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China

[2] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

ANALYST | 2013年 / 138卷 / 23期

基金：

中国国家自然科学基金;

关键词：

DOPED DIAMOND ELECTRODE; FLOW-INJECTION ANALYSIS; AMPEROMETRIC DETECTION; MONOAMINE NEUROTRANSMITTERS; CEREBROSPINAL-FLUID; MASS-SPECTROMETRY; OXIDATIVE ENZYMES; THIN-LAYER; CARBON; CATECHOLAMINES;

D O I：

10.1039/c3an01437a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Wall-jet/thin-layer amperometric electrochemical detection (ECD) coupled with high-performance liquid chromatography (HPLC) was used here for the simultaneous analysis of dopamine (DA) and homovanillic acid (HVA) at a glassy carbon electrode. Compared with the conventional thin-layer mode and wall-jet mode, the presented wall-jet/thin-layer ECD has the advantages of enhanced capture of electroactive DA and HVA on the working electrode to give enhanced responses and more convenient washing/refreshment of the working electrode surface. Under optimized conditions, the HPLC-ECD calibration curves show good linearity from 0.01 to 100 mM for DA and HVA, and the limits of detection (LODs) obtained were 1.1 nM for DA and 0.7 nM for HVA which are lower than those obtained with an UV-vis detector and a commercial electrochemical detector. The method was tested on human urine with satisfactory results. The balance of response-signal, signal-background and noise level for our HPLC-ECD system is also discussed. In addition, a demethylation electrooxidation mechanism for HVA is suggested through potentiostatic bulk electrolysis, electrospray ionization-mass spectrometry, fluorescent spectrophotometry and cyclic voltammetry studies.

引用

页码：7246 / 7253

页数：8

共 48 条

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