Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo

被引:228
作者
Czopka, Tim [1 ,2 ,3 ]
Ffrench-Constant, Charles [1 ,3 ]
Lyons, David A. [1 ,2 ]
机构
[1] Univ Edinburgh, Ctr Neuroregenerat, Edinburgh EH16 4SB, Midlothian, Scotland
[2] Univ Edinburgh, MS Soc Ctr Translat Res, Edinburgh EH16 4SB, Midlothian, Scotland
[3] Univ Edinburgh, MRC Ctr Regenerat Med, Edinburgh EH16 4UU, Midlothian, Scotland
基金
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词
AXONAL INTEGRITY; INITIAL EVENTS; ZEBRAFISH; CNS; CELLS; SYSTEM; NEURODEGENERATION; REMYELINATION; MECHANISMS; PROTEINS;
D O I
10.1016/j.devcel.2013.05.013
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The number of myelin sheaths made by individual oligodendrocytes regulates the extent of myelination, which profoundly affects central nervous system function. It remains unknown when, during their life, individual oligodendrocytes can regulate myelin sheath number in vivo. We show, using live imaging in zebrafish, that oligodendrocytes make new myelin sheaths during a period of just 5 hr, with regulation of sheath number after this time limited to occasional retractions. We also show that activation and reduction of Fyn kinase in oligodendrocytes increases and decreases sheath number per cell, respectively. Interestingly, these oligodendrocytes also generate their new myelin sheaths within the same period, despite having vastly different extents of myelination. Our data demonstrate a restricted time window relative to the lifetime of the individual oligodendrocyte, during which myelin sheath formation occurs and the number of sheaths is determined.
引用
收藏
页码:599 / 609
页数:11
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