Structural and Functional Changes of Silk Fibroin Scaffold Due to Hydrolytic Degradation

被引：40

作者：

Farokhi, Mehdi ^{[1
]}

Mottaghitalab, Fatemeh ^{[2
]}

Hadjati, Jamshid ^{[1
]}

Omidvar, Ramin ^{[3
]}

Majidi, Mohammad ^{[4
]}

Amanzadeh, Amir ^{[4
]}

Azami, Mahmoud ^{[1
]}

Tavangar, Seyed Mohammad ^{[1
]}

Shokrgozar, Mohammad Ali ^{[4
]}

Ai, Jafar ^{[1
]}

机构：

[1] Univ Tehran Med Sci, Dept Tissue Engn, Sch Adv Technol Med, Tehran 43, Iran

[2] Tarbiat Modares Univ, Fac Biol Sci, Dept Nanobiotechnol, Tehran 124, Iran

[3] Amirkabir Univ Technol, Tehran Polytech, Dept Biomed Engn, Tehran 78, Iran

[4] Pasteur Inst Iran, Natl Cell Bank Iran, Tehran 69, Iran

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2014年 / 131卷 / 06期

关键词：

IN-VITRO DEGRADATION; PHOSPHOPROTEIN COMPLEXES; COLLAGEN FIBRILS; DIFFERENTIATION; BIODEGRADATION; CALCIFICATION; PROLIFERATION; BIOMATERIALS; DELIVERY; GROWTH;

D O I：

10.1002/app.39980

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

In this study, hydrolytic degradation of silk fibroin (SF) in Phosphate buffer saline (PBS) after 12 weeks incubation was investigated. Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and X-ray diffraction (XRD) patterns have confirmed the transition from crystalline β-sheet to random coil in treated SF. A decrease in adhesion force and surface Young's modulus were observed using atomic force microscopy (AFM). Structural changes were further confirmed using scanning electron microscopy (SEM). Biocompatibility and alkaline phosphatase production of osteoblast cells were decreased significantly in treated SF scaffold. Moreover, a significant decrease in mRNA level of collagen type I and osteopontin compared with fresh SF scaffold was observed. Finally, structural and biological characteristics of SF scaffold could alter in PBS. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014, 131, 39980. Copyright © 2013 Wiley Periodicals, Inc.

引用

页数：8

共 42 条

[31] On scaffold designing for bone regeneration: A computational multiscale approach [J].