THE COMPLEX EXTRACELLULAR POLYSACCHARIDES OF MAINLY CHAIN-FORMING FRESHWATER DIATOM SPECIES FROM EPILITHIC BIOFILMS

被引:26
作者
Bahulikar, Rahul A. [1 ]
Kroth, Peter G. [1 ]
机构
[1] Univ Konstanz, Dept Biol, D-78457 Constance, Germany
关键词
diatoms; epilithic biofilms; EPS fractionation; intercellular pads; Lake Constance; lectins;
D O I
10.1111/j.1529-8817.2008.00609.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Diatoms are dominant organisms in phototrophic biofilms in aquatic habitats. They produce copious amounts of extracellular polymeric substances (EPS), which mainly consist of carbohydrates and traces of proteins and glycoproteins. This study focuses on the characterization of EPS from a total of 14 diatoms belonging to the six genera Achnanthes, Cymbella, Fragilaria, Punctastriata, Staurosira, and Pseudostaurosira, all of which were isolated from epilithic biofilms of the littoral zone of Lake Constance. EPS from all isolates were extracted by a sequential extraction procedure resulting in five different fractions. The monosaccharide composition of each fraction was analyzed by HPLC equipped with a pulse amperiometric detector, yielding results similar to those obtained by probing the EPS structures with monomer-specific fluorophore-linked lectins. Significant differences in carbohydrate composition occurred in the different fractions of single isolates. Most of the diatom isolates in our study form chain-like colonies in which the cells are attached to each other by intercellular pads. Here we demonstrate that these pads can be dissolved in hot bicarbonate and that they show a heterogeneous composition of monosaccharides in contrast to other fractions, which mostly were dominated by one or two monosaccharides. Principal component analysis indicates a correlation between carbohydrate composition of EPS fractions and the phylogenetic relationship of the respective species, indicating that EPS analyses under defined culture conditions may support taxonomic analyses.
引用
收藏
页码:1465 / 1475
页数:11
相关论文
共 45 条
[1]   Extracellular matrix assembly in diatoms (bacillariophyceae).: V.: Environmental effects on polysaccharide synthesis in the model diatom, Phaeodactylum tricornutum [J].
Abdullahi, AS ;
Underwood, GJC ;
Gretz, MR .
JOURNAL OF PHYCOLOGY, 2006, 42 (02) :363-378
[2]   Algological and bacteriological investigations on reed periphyton in Lake Velencei, Hungary [J].
Acs, É ;
Borsodi, AK ;
Makk, J ;
Molnár, P ;
Mózes, A ;
Rusznyák, A ;
Reskóne, MN ;
Kiss, KT .
HYDROBIOLOGIA, 2003, 506 (1-3) :549-557
[3]  
Acs E., 2000, Acta Bot Croat, V100, P135, DOI DOI 10.1127/ALGOL_STUD/100/2000/135
[4]  
Acs V, 1998, INT VER THEOR ANGEW, V26, P1653
[5]  
[Anonymous], 2002, Principal Component Analysis
[6]   Localization of EPS components secreted by freshwater diatoms using differential staining with fluorophore-conjugated lectins and other fluorochromes [J].
Bahulikar, Rahul A. ;
Kroth, Peter G. .
EUROPEAN JOURNAL OF PHYCOLOGY, 2007, 42 (02) :199-208
[7]   A multi-lake comparison of epilithic diatom communities on natural and artificial substrates [J].
Barbiero, RP .
HYDROBIOLOGIA, 2000, 438 (1-3) :157-170
[8]   Biofilm polymers: relationship between carbohydrate biopolymers from estuarine mudflats and unialgal cultures of benthic diatoms [J].
Bellinger, BJ ;
Abdullahi, AS ;
Gretz, MR ;
Underwood, GJC .
AQUATIC MICROBIAL ECOLOGY, 2005, 38 (02) :169-180
[9]   ISOLATION AND PARTIAL CHEMICAL-ANALYSIS OF EXOPOLYSACCHARIDES FROM THE MARINE FOULING DIATOM NAVICULA-SUBINFLATA [J].
BHOSLE, NB ;
SAWANT, SS ;
GARG, A ;
WAGH, AB .
BOTANICA MARINA, 1995, 38 (02) :103-110
[10]  
Buczko K, 1997, ACTA BOT HUNG, V40, P21