Functional study of diacylglycerol acyltransferase type 2 family in Chlamydomonas reinhardtii

被引：59
作者：
Hung, Chun-Hsien [1 ]
Ho, Ming-Yang [1 ]
Kanehara, Kazue [1 ,2 ]
Nakamura, Yuki [1 ,3 ]
机构：
[1] Acad Sinica, Inst Plant & Microbial Biol, Taipei 11529, Taiwan
[2] Muroran Inst Technol, Ctr Environm Sci & Disaster Mitigat Adv Res, Muroran, Hokkaido 050, Japan
[3] Japan Sci & Technol Agcy, PRESTO, Saitama, Japan
来源：
FEBS LETTERS | 2013年 / 587卷 / 15期
基金：
日本科学技术振兴机构;
关键词：
Algal biodiesel; Bioenergy; Triacylglycerol; Lipid droplet; Diacylglycerol acyltransferase; Chlamydomonas reinhardtii; TRIACYLGLYCEROL BIOSYNTHESIS; ENDOPLASMIC-RETICULUM; ARABIDOPSIS; GENE; EXPRESSION; ACCUMULATION; YEAST; ENZYME; DGAT1; PROTEINS;
D O I：
10.1016/j.febslet.2013.06.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Algal triacylglycerol biosynthesis is of increasing interest for potential biodiesel production. A model microalga, Chlamydomonas, has multiple isoforms of diacylglycerol acyltransferase type 2 (DGTT) catalyzing the final step of triacylglycerol biosynthesis; however, the functions of the isoforms are poorly understood. Here, we performed heterologous complementation assay of Chlamydomonas DGTT1 to 4 in a yeast mutant defective in triacylglycerol biosynthesis. DGTT1, 2 and 3 but not 4 complemented the phenotype, including triacylglycerol levels. Interestingly, complementation by DGTT2 increased triacylglycerol content by 9-fold. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2364 / 2370
页数：7
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