Quinolinic Alkaloids from Galipea longiflora Krause Suppress Production of Proinflammatory Cytokines in vitro and Control Inflammation in vivo upon Leishmania Infection in Mice

被引:14
作者
Calla-Magarinos, J. [1 ,2 ]
Quispe, T. [2 ]
Gimenez, A. [3 ]
Freysdottir, J. [4 ,5 ,6 ]
Troye-Blomberg, M. [1 ]
Fernandez, C. [1 ]
机构
[1] Stockholm Univ, Dept Immunol, Wenner Gren Inst, S-10691 Stockholm, Sweden
[2] Univ Mayor San Andres, Biochem & Pharm Fac, SELADIS Inst, Immunol Lab, La Paz, Bolivia
[3] Univ Mayor San Andres, IIFB Inst, Biochem & Pharm Fac, La Paz, Bolivia
[4] Natl Univ Hosp Iceland, Landspitali, Dept Immunol, Reykjavik, Iceland
[5] Natl Univ Hosp Iceland, Landspitali, Ctr Rheumatol Res, Reykjavik, Iceland
[6] Univ Iceland, Fac Med, Reykjavik, Iceland
关键词
FACTOR TNF-ALPHA; MUCOSAL LEISHMANIASIS; IMMUNE-RESPONSE; NITRIC-OXIDE; 2-SUBSTITUTED QUINOLINES; CUTANEOUS LEISHMANIASIS; INTERFERON-GAMMA; LESION SIZE; IFN-GAMMA; RNA GENE;
D O I
10.1111/sji.12010
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
An antileishmanial activity of quinolinic alkaloids from Galipea longiflora Krause, known as Evanta, has been demonstrated. We have previously shown that, apart from its leishmanicidal effect, in vitro pretreatment of spleen cells with an alkaloid extract of Evanta (AEE) interfered with the proliferation and interferon-? production in lymphocytes polyclonally activated either with concanavalin A or anti-CD3. In the present study, we investigated if AEE could interfere with antigen-specific lymphocyte activation. We found that in vitro and in vivo treatment reduced recall lymphocyte responses, as measured by IFN-? production (55% and 63% reduction compared to untreated cells, respectively). Apart from IFN-?, the production of IL-12 and TNF was also suppressed. No effects were observed for meglumine antimoniate (SbV), the conventional drug used to treat leishmaniasis. When mice infected with Leishmania braziliensis promastigotes in the hind footpad were treated with AEE, the dynamics of the infection changed and the footpath thickness was efficiently controlled. The parasite load was also reduced but to a lesser extent than upon treatment with SbV. Combined treatment efficiently controlled both the thickness and parasite load as smaller lesions during the entire course of the infection were seen in the mice treated with AEE plus SbV compared with AEE or SbV alone. We discuss the benefits of combined administration of AEE plus SbV.
引用
收藏
页码:30 / 38
页数:9
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