Rapid fluorescent assay for screening drugs on Leishmania amastigotes

被引：54

作者：

Shimony, Orly ^{[1
]}

Jaffe, Charles L. ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Parasitol, Kuvin Ctr Study Infect & Trop Dis, IL-91220 Jerusalem, Israel

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2008年 / 75卷 / 02期

关键词：

AlamarBlue; Leishmania donovani; Leishmania tropica; Amphotericin B; Staurosporine; Protein kinase inhibitors;

D O I：

10.1016/j.mimet.2008.05.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid fluorescent viability assay employing alamarBlue was optimized for use with Leishmania axenic amastigotes, the stage of the parasite responsible for disease pathology. The activity of two protein kinase inhibitors, Staurosporine and H-89, as well as Amphotericin B, on promastigotes and amastigotes of Leishmania donovani and Leishmania tropica was compared. Both protein kinase inhibitors inhibited promastigote growth at lower concentrations than amastigotes, while the GI(50) for Amphotericin B on both stages was similar. This assay only requires a limited number of axenic amastigotes (50,000 cells/well) and can be used to rapidly screen large chemical or natural product libraries for activity against amastigotes. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：196 / 200

页数：5

共 29 条

[21] Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity [J].

O'Brien, J ;

Wilson, I ;

Orton, T ;

Pognan, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (17) :5421-5426

[22] Rapid drug resistance detection in Mycobacterium tuberculosis:: a review of colourimetric methods [J].

Palomino, J. C. ;

Martin, A. ;

Portaels, F. .

CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (08) :754-762

[23] The Alamar Blue(R) assay to determine drug sensitivity of African trypanosomes (T-b-rhodesiense and T-b-gambiense) in vitro [J].

Raz, B ;

Iten, M ;

GretherBuhler, Y ;

Kaminsky, R ;

Brun, R .

ACTA TROPICA, 1997, 68 (02) :139-147

[24] 2-(3-aryl-3-oxopropen-1-yl)-9-tert-butyl-paullones:: A new antileishmanial chemotype [J].

Reichwald, Christina ;

Shimony, Orly ;

Dunkel, Ute ;

Sacerdoti-Sierra, Nina ;

Jaffe, Charles L. ;

Kunick, Conrad .

JOURNAL OF MEDICINAL CHEMISTRY, 2008, 51 (03) :659-665

[25] Development of resazurin microtiter assay for drug sensibility testing of Trypanosoma cruzi epimastigotes [J].

Rolón, Miriam ;

Vega, Celeste ;

Escario, José A. ;

Gómez-Barrio, Alicia .

PARASITOLOGY RESEARCH, 2006, 99 (02) :103-107

[26] Advances and perspectives in Leishmania cell based drug-screening procedures [J].

Sereno, D. ;

da Silva, A. Cordeiro ;

Mathieu-Daude, F. ;

Ouaissi, A. .

PARASITOLOGY INTERNATIONAL, 2007, 56 (01) :3-7

[27] Axenically cultured amastigote forms as an in vitro model for investigation of antileishmanial agents [J].

Sereno, D ;

Lemesre, JL .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (05) :972-976

[28] Characterization of the anti-Leishmania effect induced by cisplatin, an anticancer drug [J].

Tavares, J. ;

Ouaissi, M. ;

Ouaissi, A. ;

Cordeiro-da-Silva, A. .

ACTA TROPICA, 2007, 103 (02) :133-141

[29]

World Health Organization, 2007, LEISHM

← 1 2 3 →