Action Potential Clamp and Pharmacology of the Variant 1 Short QT Syndrome T618I hERG K+ Channel

被引:17
作者
El Harchi, Aziza [1 ,2 ]
Melgari, Dario [1 ,2 ]
Zhang, Yi Hong [1 ,2 ]
Zhang, Henggui [3 ]
Hancox, Jules C. [1 ,2 ]
机构
[1] Univ Bristol, Sch Physiol & Pharmacol, Bristol, Avon, England
[2] Univ Bristol, Cardiovasc Res Labs, Bristol, Avon, England
[3] Univ Manchester, Sch Phys & Astron, Biol Phys Grp, Manchester, Lancs, England
来源
PLOS ONE | 2012年 / 7卷 / 12期
关键词
POTASSIUM CHANNEL; MOLECULAR DETERMINANTS; DOFETILIDE BLOCK; CLASS-I; MUTATION; INHIBITION; RECTIFIER; DISOPYRAMIDE; DEPENDENCE; QUINIDINE;
D O I
10.1371/journal.pone.0052451
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: The familial Short QT Syndrome (SQTS) is associated with an increased risk of cardiac arrhythmia and sudden death. Gain-of-function mutations in the hERG K+ channel protein have been linked to variant 1 of the SQTS. A hERG channel pore (T618I) mutation has recently been identified in families with heritable SQTS. This study aimed to determine effects of the T618I-hERG mutation on (i) hERG current (I-hERG) elicited by ventricular action potentials; (ii) the sensitivity of I-hERG to inhibition by four clinically used antiarrhythmic drugs. Methods: Electrophysiological recordings of I-hERG were made at 37 degrees C from HEK 293 cells expressing wild-type (WT) or T618I hERG. Whole-cell patch clamp recording was performed using both conventional voltage clamp and ventricular action potential (AP) clamp methods. Results: Under conventional voltage-clamp, WT I-hERG peaked at 0-+10 mV, whilst for T618I I-hERG maximal current was rightward shifted to similar to +40 mV. Voltage-dependent activation and inactivation of T618I I-hERG were positively shifted (respectively by +15 and similar to +25 mV) compared to WT I-hERG. The I-hERG 'window' was increased for T618I compared to WT hERG. Under ventricular AP clamp, maximal repolarising WT I-hERG occurred at similar to -30 mV, whilst for T618I hERG peak I-hERG occurred earlier during AP repolarisation, at similar to +5 mV. Under conventional voltage clamp, half-maximal inhibitory concentrations (IC50) for inhibition of I-hERG tails by quinidine, disopyramide, D-sotalol and flecainide for T618I hERG ranged between 1.4 and 3.2 fold that for WT hERG. Under action potential voltage clamp, T618I IC(50)s ranged from 1.2 to 2.0 fold the corresponding IC50 values for WT hERG. Conclusions: The T618I mutation produces a more modest effect on repolarising I-hERG than reported previously for the N588K-hERG variant 1 SQTS mutation. All drugs studied here appear substantially to retain their ability to inhibit I-hERG in the setting of the SQTS-linked T618I mutation.
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页数:15
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