Metabolomic Study of Urine from Workers Exposed to Low Concentrations of Benzene by UHPLC-ESI-QToF-MS Reveals Potential Biomarkers Associated with Oxidative Stress and Genotoxicity

被引:13
作者
Mendes, Michele P. R. [1 ]
Paiva, Maria Jose N. [1 ]
Costa-Amaral, Isabele C. [2 ]
Carvalho, Leandro V. B. [2 ]
Figueiredo, Victor O. [2 ]
Goncalves, Eline S. [2 ]
Larentis, Ariane L. [2 ]
Andre, Leiliane C. [1 ]
机构
[1] Fed Univ Minas Gerais UFMG, Fac Pharm, Dept Clin & Toxicol Anal, BR-31270901 Belo Horizonte, MG, Brazil
[2] Fundacao Oswaldo Cruz, Ctr Study Occupat Hlth & Human Ecol CESTEH, Sergio Arouca Natl Sch Publ Hlth ENSP, FIOCRUZ, Rua Leopoldo Bulhoes 1480, BR-21041210 Manguinho, RJ, Brazil
关键词
benzene; metabolomic untargeted; UHPLC-ESI-Q-TOF-MS; environmental toxicology; occupational toxicology; CHROMATOGRAPHY-MASS-SPECTROMETRY; SPHINGOLIPID METABOLISM; INDUCED MYELOTOXICITY; BONE-MARROW; CANCER; PLASMA; MICE; INFLAMMATION; METABONOMICS; STRATEGIES;
D O I
10.3390/metabo12100978
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Benzene is a human carcinogen whose exposure to concentrations below 1 ppm (3.19 mg.m(-3)) is associated with myelotoxic effects. The determination of biomarkers such as trans-trans muconic acid (AttM) and S-phenylmercapturic acid (SPMA) show exposure without reflecting the toxic effects of benzene. For this reason, in this study, the urinary metabolome of individuals exposed to low concentrations of benzene was investigated, with the aim of understanding the biological response to exposure to this xenobiotic and identifying metabolites correlated with the toxic effects induced by it. Ultra-efficient liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometer (UHPLC-ESI-Q-ToF-MS) was used to identify metabolites in the urine of environmentally (n = 28) and occupationally exposed (n = 32) to benzene (mean of 22.1 mu g.m(-3) and 31.8 mu g.m(-3), respectively). Non-targeted metabolomics analysis by PLS-DA revealed nine urinary metabolites discriminating between groups and statistically correlated with oxidative damage (MDA, thiol) and genetic material (chromosomal aberrations) induced by the hydrocarbon. The analysis of metabolic pathways revealed important alterations in lipid metabolism. These results point to the involvement of alterations in lipid metabolism in the mechanisms of cytotoxic and genotoxic action of benzene. Furthermore, this study proves the potential of metabolomics to provide relevant information to understand the biological response to exposure to xenobiotics and identify early effect biomarkers.
引用
收藏
页数:29
相关论文
共 93 条
[11]  
Canuto GAB, 2018, QUIM NOVA, V41, P75
[12]   Biological monitoring of low level exposure to benzene in an oil refinery: Effect of modulating factors [J].
Carrieri, Mariella ;
Spatari, Giovanna ;
Tranfo, Giovanna ;
Sapienza, Daniela ;
Scapellato, Maria Luisa ;
Bartolucci, Giovanni Battista ;
Manno, Maurizio .
TOXICOLOGY LETTERS, 2018, 298 :70-75
[13]   Metabolomic analysis of the toxic effect of chronic exposure of cadmium on rat urine [J].
Chen, Shuai ;
Zhang, Meiyan ;
Bo, Lu ;
Li, Siqi ;
Hu, Liyan ;
Zhao, Xiujuan ;
Sun, Changhao .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2018, 25 (04) :3765-3774
[14]  
Chong Jasmine, 2019, Curr Protoc Bioinformatics, V68, pe86, DOI 10.1002/cpbi.86
[15]   Environmental Assessment and Evaluation of Oxidative Stress and Genotoxicity Biomarkers Related to Chronic Occupational Exposure to Benzene [J].
Costa-Amaral, Isabele C. ;
Carvalho, Leandro V. B. ;
Santos, Marcus Vinicius C. ;
Valente, Daniel ;
Pereira, Angelica C. ;
Figueiredo, Victor O. ;
de Souza, Juliana Mendonca ;
Castro, Vinicio S. ;
Trancoso, Maria de Fatima ;
Fonseca, Antonio Sergio A. ;
Milagres, Vanessa G. ;
Mendes, Michele P. R. ;
Paiva, Maria Jose N. ;
Andre, Leiliane C. ;
Borges, Renato M. ;
Menezes, Marco Antonio C. ;
Alves, Sergio R. ;
Goncalves, Eline S. ;
Sisenando, Herbert Ary ;
Perini, Jamila A. ;
Oliveira, Monica S. ;
Moura-Correa, Maria Juliana ;
Teixeira, Liliane R. ;
Collins, Andrew R. ;
Mattos, Rita de Cassia O. C. ;
Sarcinelli, Paula N. ;
Larentis, Ariane L. .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2019, 16 (12)
[16]  
Cummings J.H., 1995, PHYSL CLIN ASPECTS S
[17]   Hematological and hepatic alterations in nonsmoking residents exposed to benzene following a flaring incident at the British petroleum plant in Texas City [J].
D'Andrea, Mark A. ;
Reddy, G. Kesava .
ENVIRONMENTAL HEALTH, 2014, 13
[18]  
de Moraes Moreau R.L., 2016, TOXICOLOGIA ANAL, V2nd
[19]   NMR Spectroscopy for Metabolomics Research [J].
Emwas, Abdul-Hamid ;
Roy, Raja ;
McKay, Ryan T. ;
Tenori, Leonardo ;
Saccenti, Edoardo ;
Gowda, G. A. Nagana ;
Raftery, Daniel ;
Alahmari, Fatimah ;
Jaremko, Lukasz ;
Jaremko, Mariusz ;
Wishart, David S. .
METABOLITES, 2019, 9 (07)
[20]   Effects of systemic prostaglandin E1 on splanchnic and peripheral haemodynamics in control subjects and in patients with cirrhosis [J].
Fabbri, A ;
Magalotti, D ;
Marchesini, G ;
Brizi, M ;
Bianchi, G ;
Zoli, M .
PROSTAGLANDINS & OTHER LIPID MEDIATORS, 1998, 55 (04) :209-218