Chemometric-assisted method development in reversed-phase liquid chromatography

被引：51

作者：

Cela, R. ^{[1
]}

Ordonez, E. Y. ^{[1
]}

Quintana, J. B. ^{[1
]}

Rodil, R. ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Dept Analyt Chem & Food Sci, Inst Res Food Anal, Santiago De Compostela, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1287卷

关键词：

Chromatographic optimization; Computerized separations; Chemometrics in chromatography; ARTIFICIAL NEURAL-NETWORKS; OFF-LINE OPTIMIZATION; RESTRICTED MULTIPARAMETER APPROACH; MULTILINEAR GRADIENT ELUTION; MASS SPECTROMETRY; COLUMN SELECTIVITY; MOBILE-PHASE; BACKGROUND REDUCTION; PROGRAMMED ELUTIONS; COMPONENT DETECTION;

D O I：

10.1016/j.chroma.2012.07.081

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The computer-assisted development of separation procedures in liquid chromatography (LC), which has involved the adaptation of diverse chemometric techniques and the incorporation of successive advances in informatics and computing over a period of more than four decades, has provided chromatographers with commercial and academic tools of great utility. These tools enable the development, optimization (including robustness studies) and transfer of procedures that constitute one of the analytical activities most commonly used at present in laboratories and industries worldwide. Departing from a typical operational scheme of manually developed chromatographic procedures, this review describes some of the different tools and options that chemometrics provides to the chromatographer for the computer-assisted approach, and outlines the present status of this approach and possible reasons why it is still not considered fully satisfactory. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：2 / 22

页数：21

共 112 条

[31]

Euerby M.R., 2000, LC GC EUR, V13, P665

[32] Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns containing phenyl moieties using principal component analysis [J].