Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa

被引：11
作者：
Sánchez, A
De la Casa, RM
Sinisterra, JV
Valero, F [1 ]
Sánchez-Montero, JM
机构：
[1] Univ Autonoma Barcelona, Dept Chem Engn, E-08193 Barcelona, Spain
[2] Univ Complutense Madrid, Dept Organ & Pharmaceut Chem, E-28040 Madrid, Spain
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1999年 / 80卷 / 01期
关键词：
Candida rugosa lipase; downstream; isoenzymes; lipolytic activity; esterification; Ketoprofen;
D O I：
10.1385/ABAB:80:1:65
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Different fed-batch cultures of Candida rugosa were carried out using oleic acid as the only carbon source. The crude lipases obtained under several operational conditions and downstream processes showed different catalytic activity and isoenzymes ratio. This fact implied that the performance of the lipase produced could be modulated by using different operational fermentation conditions. These powders were compared with commercial lipase from Sigma (St. Louis, MO) in hydrolysis and synthesis reactions. Especially interesting was the fact that the enantioselectivity of a crude lipase was higher than that observed with commercial lipase in the resolution of racemic Ketoprofen. In addition, response of both lipases in the presence of water was different.
引用
收藏
页码：65 / 75
页数：11
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