Comparative transcriptome analyses in contrasting onion (Allium cepa L.) genotypes for drought stress

被引：38

作者：

Ghodke, Pranjali ^{[1
]}

Khandagale, Kiran ^{[1
]}

Thangasamy, A. ^{[1
]}

Kulkarni, Abhijeet ^{[2
]}

Narwade, Nitin ^{[2
]}

Shirsat, Dhananjay ^{[1
]}

Randive, Pragati ^{[1
]}

Roylawar, Praveen ^{[3
]}

Singh, Isha ^{[4
]}

Gawande, Suresh J. ^{[1
]}

Mahajan, Vijay ^{[1
]}

Solanke, Amolkumar ^{[5
]}

Singh, Major ^{[1
]}

机构：

[1] ICAR Directorate Onion & Garl Res, Pune, Maharashtra, India

[2] Savitribai Phule Pune Univ, Dept Bioinformat, Pune, Maharashtra, India

[3] SN Arts DJM Commerce & BNS Sci Coll, Sangamner, India

[4] Univ Coll, Sch Biomol Sci, Dublin, Ireland

[5] ICAR Natl Inst Plant Biotechnol, New Delhi, India

来源：

PLOS ONE | 2020年 / 15卷 / 08期

关键词：

ABIOTIC STRESS; GENE-EXPRESSION; WATER-STRESS; TOLERANCE; ARABIDOPSIS; TRANSPORTER; EXTRACTION; ENZYMES; PROTEIN; YIELD;

D O I：

10.1371/journal.pone.0237457

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Onion (Allium cepaL.) is an important vegetable crop widely grown for diverse culinary and nutraceutical properties. Being a shallow-rooted plant, it is prone to drought. In the present study, transcriptome sequencing of drought-tolerant (1656) and drought-sensitive (1627) onion genotypes was performed to elucidate the molecular basis of differential response to drought stress. A total of 123206 and 139252 transcripts (average transcript length: 690 bases) were generated after assembly for 1656 and 1627, respectively. Differential gene expression analyses revealed upregulation and downregulation of 1189 and 1180 genes, respectively, in 1656, whereas in 1627, upregulation and downregulation of 872 and 1292 genes, respectively, was observed. Genes encoding transcription factors, cytochrome P450, membrane transporters, and flavonoids, and those related to carbohydrate metabolism were found to exhibit a differential expression behavior in the tolerant and susceptible genotypes. The information generated can facilitate a better understanding of molecular mechanisms underlying drought response in onion.

引用

页数：21

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