An Amphiphilic Dendrimer for Effective Delivery of Small Interfering RNA and Gene Silencing In Vitro and In Vivo

被引：215

作者：

Yu, Tianzhu ^{[1
,2
]}

Liu, Xiaoxuan ^{[2
,3
]}

Bolcato-Bellemin, Anne-Laure ^{[4
]}

Wang, Yang ^{[1
]}

Liu, Cheng

Erbacher, Patrick ^{[4
]}

Qu, Fanqi ^{[2
]}

Rocchi, Palma ^{[3
]}

Behr, Jean-Paul ^{[5
]}

Peng, Ling ^{[1
]}

机构：

[1] Aix Marseille Univ, Ctr Interdisciplinaire Nanosci Marseille, CNRS, CINaM,UMR 7325, F-13288 Marseille, France

[2] Wuhan Univ, Coll Chem & Mol Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

[3] Aix Marseille Univ, Ctr Rech Cancerol Marseille, Inst Paoli Calmettes, CNRS,INSERM,U1068,UMR 7258, F-13009 Marseille, France

[4] Polyplus Transfect SA, F-67401 Illkirch Graffenstaden, France

[5] Univ Strasbourg, Fac Pharm, Lab Chim Genet, CNRS,UMR7199, F-67401 Illkirch Graffenstaden, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 34期

关键词：

dendrimers; drug design; gene silencing; prostate cancer; siRNA; DENDRON-BEARING LIPIDS; PROSTATE-CANCER; SIRNA DELIVERY; PAMAM DENDRIMERS; ANDROGEN ABLATION; VECTORS; HSP27; GENERATION; KNOCKING; BARRIERS;

D O I：

10.1002/anie.201203920

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An amphiphilic dendrimer bearing a hydrophobic alkyl chain and hydrophilic poly(amidoamine) dendrons is able to combine the advantageous features of lipid and dendrimer vectors to deliver a heat shock protein?27 siRNA and produce potent gene silencing and anticancer activity in?vitro and in?vivo in a prostate cancer model (see picture). This dendrimer can be used alternatively for treating various diseases.

引用

页码：8478 / 8484

页数：7

共 33 条

[1] Small heat shock proteins HSP27 (HspB1), αB-crystallin (HspB5) and HSP22 (HspB8) as regulators of cell death [J].

Acunzo, Julie ;

Katsogiannou, Maria ;

Rocchi, Palma .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2012, 44 (10) :1622-1631

[2] Heat shock factors: integrators of cell stress, development and lifespan [J].

Akerfelt, Malin ;

Morimoto, Richard I. ;

Sistonen, Lea .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2010, 11 (08) :545-555

[3]

[Anonymous], ANGEW CHEM

[4] Hsp27 (HspB1) and αB-crystallin (HspB5) as therapeutic targets [J].

Arrigo, Andr-Patrick ;

Simon, Stphanie ;

Gibert, Benjamin ;

Kretz-Remy, Carole ;

Nivon, Mathieu ;

Czekalla, Anna ;

Guillet, Dominique ;

Moulin, Maryline ;

Diaz-Latoud, Chantal ;

Vicart, Patrick .

FEBS LETTERS, 2007, 581 (19) :3665-3674

[5] Degradable Self-Assembling Dendrons for Gene Delivery: Experimental and Theoretical Insights into the Barriers to Cellular Uptake [J].

Barnard, Anna ;

Posocco, Paola ;

Pricl, Sabrina ;

Calderon, Marcelo ;

Haag, Rainer ;

Hwang, Mark E. ;

Shum, Victor W. T. ;

Pack, Daniel W. ;

Smith, David K. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (50) :20288-20300

[6] Versatile peptide dendrimers for nucleic acid delivery [J].

Bayele, HK ;

Sakthivel, T ;

O'Donell, M ;

Pasi, KJ ;

Wilderspin, AF ;

Lee, CA ;

Toth, I ;

Florence, AT .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2005, 94 (02) :446-457

[7] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[8] The promises and pitfalls of RNA-interference-based therapeutics [J].

Castanotto, Daniela ;

Rossi, John J. .

NATURE, 2009, 457 (7228) :426-433

[9] THE ROLE OF DIOLEOYL PHOSPHATIDYLETHANOLAMINE IN CATIONIC LIPOSOME-MEDIATED GENE-TRANSFER [J].

FARHOOD, H ;

SERBINA, N ;

HUANG, L .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1235 (02) :289-295

[10] Amphiphilic dendrimers: Novel self-assembling vectors for efficient gene delivery [J].

Joester, D ;

Losson, M ;

Pugin, R ;

Heinzelmann, H ;

Walter, E ;

Merkle, HP ;

Diederich, F .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (13) :1486-1490

← 1 2 3 4 →