Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat

被引：63

作者：

Castonguay-Paradis, Sophie ^{[1
,3
,6
]}

Lacroix, Sebastien ^{[1
,6
]}

Rochefort, Gabrielle ^{[1
,3
,6
]}

Parent, Lydiane ^{[3
,6
]}

Perron, Julie ^{[1
,6
]}

Martin, Cyril ^{[2
,6
]}

Lamarche, Benoit ^{[1
,3
]}

Raymond, Frederic ^{[1
,3
,6
]}

Flamand, Nicolas ^{[2
,4
,6
]}

Di Marzo, Vincenzo ^{[1
,2
,3
,5
,6
]}

Veilleux, Alain ^{[1
,3
,6
]}

机构：

[1] Univ Laval, Ctr Nutr Sante & Soc NUTRISS, INAF, 2440,Blvd Hochelaga, Quebec City, PQ G1V 0A6, Canada

[2] Univ Laval, Ctr Rech, IUCPQ, Quebec City, PQ, Canada

[3] Univ Laval, FSAA, Ecole Nutr, Quebec City, PQ, Canada

[4] Univ Laval, Dept Med, Fac Med, Quebec City, PQ, Canada

[5] Joint Int Unit Chem & Biomol Res Microbiome & Its, Pozzuoli, Italy

[6] Univ Laval, Canada Res Excellence Chair, Microbiome Endocannabinoidome Axis Metab Hlth CER, Quebec City, PQ, Canada

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

SYSTEM; ACTIVATION; SECRETION; GPR119; PLASMA; MICE;

D O I：

10.1038/s41598-020-72861-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The endocannabinoidome encompasses several fatty acid (FA)-derived mediators, including the endocannabinoid anandamide (AEA) and 2-arachidonoyl-glycerol (2-AG), which served as targets for anti-obesity drug development, and their congener N-acyl-ethanolamines (NAEs) and 2-monoacyl-glycerols (2-MAGs), which are involved in food intake and energy metabolism. Body weight and fat distribution have been suggested as determinants of peripheral endocannabinoid levels. We aimed at investigating factors, beyond body fat composition, that are associated with circulating NAE and 2-MAG levels in a heterogeneous human population. Plasma NAEs and 2-MAGs were measured using LC-MS/MS in a cross-sectional sample of healthy men and women (n=195) covering a wide range of BMI and individuals before and after a 2-day Mediterranean diet (n=21). Circulating levels of all 2-MAGs and NAEs, other than N-oleoyl-ethanolamine (OEA), correlated with body fat mass and visceral adipose tissue (0.26<r<0.54). NAE levels were elevated in individuals with elevated fat mass, while 2-MAGs were increased in individuals with predominantly visceral body fat distribution. Dietary intakes of specific FAs were associated with 2-AG and omega-3-FA-derived NAEs or 2-MAGs, irrespective of the body fat distribution. Some gut bacterial families (e.g. Veillonellaceae, Peptostreptococcaceae and Akkermansiaceae) were associated with variations in most NAEs or omega-3-FA-derived 2-MAGs, independently of fat mass and dietary FA intake. Finally, a 2-day Mediterranean diet intervention increased circulating levels of NAEs and 2-MAGs in agreement with changes in FA intake (p<0.01). Self-reported intake and short-term dietary intervention increased in oleic acid and EPA and DHA intake as well as certain gut microbiota taxa are associated to circulating NAEs and 2-MAGs independently of adiposity measures, thus highlighting the potential importance of these variables in determining endocannabinoidome signaling in humans.

引用

页数：11

共 56 条

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