Expression of mRNA for 5-HT2 Receptors and Proteins Related to Inactivation of 5-HT in Mouse Osteoblasts

被引:16
作者
Hirai, Takao [1 ]
Tokumo, Kohji [1 ]
Tsuchiya, Daijyu [1 ]
Nishio, Hiroaki [1 ]
机构
[1] Fukuyama Univ, Fac Pharm & Pharmaceut Sci, Dept Mol Pharmacol, Hiroshima 7290292, Japan
关键词
osteoblast; 5-HT2; receptor; 5-HT transporter (5-HTT); RAT HIPPOCAMPAL-NEURONS; SEROTONIN; 5-HYDROXYTRYPTAMINE; BONE; TRANSPORTER; REUPTAKE;
D O I
10.1254/jphs.08243SC
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
We analyzed the expression of 5-HT2 receptors and proteins related to inactivation of 5-HT in primary cultures of mouse osteoblasts. The mRNA for the 5-HT2A receptor was detectable in anaplastic osteoblasts as well as in differentiated and matured osteoblasts. The mRNA for the 5-HT2B receptor and 5-HT transporter was undetectable in anaplastic osteoblasts and became detectable in differentiated and matured osteoblasts. It was suggested that 5-HT might regulate the proliferation of anaplastic osteoblasts; through the 5-HT2A receptor without control by 5-HT-inactivating mechanisms. The differentiation and maturation of osteoblasts might be regulated by the activation of the 5-HT2B receptor under the control of 5-HT inactivation.
引用
收藏
页码:319 / 323
页数:5
相关论文
共 14 条
  • [1] Serotonin transporter and receptor expression in osteocytic MLO-Y4 cells
    Bliziotes, M.
    Eshleman, A.
    Burt-Pichat, B.
    Zhang, X. -W.
    Hashimoto, J.
    Wiren, K.
    Chenu, C.
    [J]. BONE, 2006, 39 (06) : 1313 - 1321
  • [2] Neurotransmitter action in osteoblasts: Expression of a functional system for serotonin receptor activation and reuptake
    Bliziotes, MM
    Eshleman, AJ
    Zhang, XW
    Wiren, KM
    [J]. BONE, 2001, 29 (05) : 477 - 486
  • [3] Various effects of antidepressant drugs on bone microarchitectecture, mechanical properties and bone remodeling
    Bonnet, N.
    Bernard, P.
    Beaupied, H.
    Bizot, J. C.
    Trovero, F.
    Courteix, D.
    Benhamou, C. L.
    [J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2007, 221 (01) : 111 - 118
  • [4] The serotonin 5-HT2B receptor controls bone mass via osteoblast recruitment and proliferation
    Collet, C.
    Schiltz, C.
    Geoffroy, V.
    Maroteaux, L.
    Launay, J. -M.
    de Vernejoul, M. -C.
    [J]. FASEB JOURNAL, 2008, 22 (02) : 418 - 427
  • [5] Transactivation of core binding factor α1 as a basic mechanism to trigger parathyroid hormone-induced osteogenesis
    Fujita, T
    Fukuyama, R
    Izumo, N
    Hirai, T
    Meguro, T
    Nakamuta, H
    Koida, M
    [J]. JAPANESE JOURNAL OF PHARMACOLOGY, 2001, 86 (04) : 405 - 416
  • [6] Serotonin and fluoxetine modulate bone cell function in vitro
    Gustafsson, BI
    Thommesen, L
    Stunes, AK
    Tommeras, K
    Westbroek, I
    Waldum, HL
    Slordahl, K
    Tamburstuen, MV
    Reseland, JE
    Syversen, U
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 98 (01) : 139 - 151
  • [7] Long-term serotonin administration leads to higher bone mineral density, affects bone architecture, and leads to higher femoral bone stiffness in rats
    Gustafsson, BI
    Westbroek, I
    Waarsing, JH
    Waldum, H
    Solligård, E
    Brunsvik, A
    Dimmen, S
    van Leeuwen, JPTM
    Weinans, H
    Syversen, U
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 97 (06) : 1283 - 1291
  • [8] Stimulation of ubiquitin-proteasome pathway through the expression of amidohydrolase for N-terminal asparagine (Ntan1) in cultured rat hippocampal neurons exposed to static magnetism
    Hirai, T
    Taniura, H
    Goto, Y
    Ogura, M
    Sng, JCG
    Yoneda, Y
    [J]. JOURNAL OF NEUROCHEMISTRY, 2006, 96 (06) : 1519 - 1530
  • [9] Functional alterations in immature cultured rat hippocampal neurons after sustained exposure to static magnetic fields
    Hirai, T
    Yoneda, Y
    [J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2004, 75 (02) : 230 - 240
  • [10] Effect of selective serotonin reuptake inhibitors on the risk of fracture
    Richards, J. Brent
    Papaioannou, Alexandra
    Adachi, Jonathan D.
    Joseph, Lawrence
    Whitson, Heather E.
    Prior, Jerilynn C.
    Goltzman, David
    [J]. ARCHIVES OF INTERNAL MEDICINE, 2007, 167 (02) : 188 - 194