Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis

被引:103
作者
Jin, Miao
Jiang, Yaoming
Sun, Linlin
Yin, Jianhua
Fu, Huihui
Wu, Genfu
Gao, Haichun [1 ]
机构
[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310003, Zhejiang, Peoples R China
来源
PLOS ONE | 2013年 / 8卷 / 09期
基金
中国国家自然科学基金;
关键词
NITRITE REDUCTASE; DIMETHYL-SULFOXIDE; HEME-C; MR-1; IDENTIFICATION; RESPIRATION; BIOGENESIS; ATTACHMENT; EXPRESSION; ELEMENTS;
D O I
10.1371/journal.pone.0075610
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c-type cytochrome mutants utilizing a newly developed att-based mutagenesis approach, which is more effective and efficient than the approach used previously by circumventing the conventional cloning. Second, we investigated the cytochrome c maturation (Ccm) system in S. oneidensis. There are two loci predicted to encode components of the Ccm system, SO0259-SO0269 and SO0476-SO0478. The former is proven essential for cytochrome c maturation whereas the latter is dispensable. Unlike the single operon organization observed in other gamma-proteobacteria, genes at the SO0259-SO0269 locus are uniquely organized into four operons, ccmABCDE, scyA, SO0265, and ccmFGH-SO0269. Functional analysis revealed that the SO0265 gene rather than the scyA and SO0269 genes are relevant to cytochrome c maturation.
引用
收藏
页数:10
相关论文
共 43 条
  • [1] An octaheme c-type cytochrome from Shewanella oneidensis can reduce nitrite and hydroxylamine
    Atkinson, Sally J.
    Mowat, Christopher G.
    Reid, Graeme A.
    Chapman, Stephen K.
    [J]. FEBS LETTERS, 2007, 581 (20) : 3805 - 3808
  • [2] Global transcriptome analysis of Shewanella oneidensis MR-1 exposed to different terminal electron acceptors
    Beliaev, AS
    Klingeman, DM
    Klappenbach, JA
    Wu, L
    Romine, MF
    Tiedje, JA
    Nealson, KH
    Fredrickson, JK
    Zhou, J
    [J]. JOURNAL OF BACTERIOLOGY, 2005, 187 (20) : 7138 - 7145
  • [3] SIMULTANEOUS DETERMINATION OF HEMES-A, HEMES-B, AND HEMES-C FROM PYRIDINE HEMOCHROME SPECTRA
    BERRY, EA
    TRUMPOWER, BL
    [J]. ANALYTICAL BIOCHEMISTRY, 1987, 161 (01) : 1 - 15
  • [4] Current production and metal oxide reduction by Shewanella oneidensis MR-1 wild type and mutants
    Bretschger, Orianna
    Obraztsova, Anna
    Sturm, Carter A.
    Chang, In Seop
    Gorby, Yuri A.
    Reed, Samantha B.
    Culley, David E.
    Reardon, Catherine L.
    Barua, Soumitra
    Romine, Margaret F.
    Zhou, Jizhong
    Beliaev, Alexander S.
    Bouhenni, Rachida
    Saffarini, Daad
    Mansfeld, Florian
    Kim, Byung-Hong
    Fredrickson, James K.
    Nealson, Kenneth H.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (21) : 7003 - 7012
  • [5] Impact of the bacterial type I cytochrome c maturation system on different biological processes
    Cianciotto, NP
    Cornelis, P
    Baysse, C
    [J]. MOLECULAR MICROBIOLOGY, 2005, 56 (06) : 1408 - 1415
  • [6] Involvement of products of the nrfEFG genes in the covalent attachment of haem c to a novel cysteine-lysine motif in the cytochrome c552 nitrite reductase from Escherichia coli
    Eaves, DJ
    Grove, J
    Staudenmann, W
    James, P
    Poole, RK
    White, SA
    Griffiths, I
    Cole, JA
    [J]. MOLECULAR MICROBIOLOGY, 1998, 28 (01) : 205 - 216
  • [7] STRUCTURE AND FUNCTION OF FORMATE-DEPENDENT CYTOCHROME C NITRITE REDUCTASE, NRFA
    Einsle, Oliver
    [J]. METHODS IN ENZYMOLOGY, VOL 46: RESEARCH ON NITRIFICATION AND RELATED PROCESSES, PT B, 2011, 496 : 399 - 422
  • [8] Mind the gap: cytochrome interactions reveal electron pathways across the periplasm of Shewanella oneidensis MR-1
    Fonseca, Bruno M.
    Paquete, Catarina M.
    Neto, Sonia E.
    Pacheco, Isabel
    Soares, Claudio M.
    Louro, Ricardo O.
    [J]. BIOCHEMICAL JOURNAL, 2013, 449 : 101 - 108
  • [9] Towards environmental systems biology of Shewanella
    Fredrickson, James K.
    Romine, Margaret F.
    Beliaev, Alexander S.
    Auchtung, Jennifer M.
    Driscoll, Michael E.
    Gardner, Timothy S.
    Nealson, Kenneth H.
    Osterman, Andrei L.
    Pinchuk, Grigoriy
    Reed, Jennifer L.
    Rodionov, Dmitry A.
    Rodrigues, Jorge L. M.
    Saffarini, Daad A.
    Serres, Margrethe H.
    Spormann, Alfred M.
    Zhulin, Igor B.
    Tiedje, James M.
    [J]. NATURE REVIEWS MICROBIOLOGY, 2008, 6 (08) : 592 - 603
  • [10] Structure of a trypanosomatid mitochondrial cytochrome c with heme attached via only one thioether bond and implications for the substrate recognition requirements of heme lyase
    Fueloep, Vilmos
    Sam, Katharine A.
    Ferguson, Stuart J.
    Ginger, Michael L.
    Allen, James W. A.
    [J]. FEBS JOURNAL, 2009, 276 (10) : 2822 - 2832