A xanthine oxidase colloidal gold enzyme electrode for amperometric biosensor applications

被引：108
作者：
Zhao, JG
ODaly, JP
Henkens, RW
Stonehuerner, J
Crumbliss, AL
机构：
[1] DUKE UNIV,DEPT CHEM,DURHAM,NC 27708
[2] ANDCARE INC,DURHAM,NC 27713
来源：
BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 05期
关键词：
xanthine oxidase; enzyme electrode; bioelectrode; biosensor; colloidal gold; xanthine; hypoxanthine;
D O I：
10.1016/0956-5663(96)86786-8
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
An electrode has been prepared based on xanthine oxidase adsorbed to colloidal gold and evaporated onto the surface of glassy carbon. This electrode responds to xanthine or hypoxanthine in the absence of added mediator by electrochemical oxidation of the enzymatic oxidation product, uric acid, at the electrode surface. The electrode can also be used in the presence of an electron transfer mediator to detect other substrates for xanthine oxidase such as 4-hydroxypyrimidine.
引用
收藏
页码：493 / 502
页数：10
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