Use of Solid Phase Microextraction (SPME) for Profiling the Volatile Metabolites Produced by Glomerella cingulata

被引:13
作者
Miyazawa, Mitsuo [1 ]
Kimura, Minako [1 ]
Yabe, Yoshito [2 ]
Tsukamoto, Daisuke [2 ]
Sakamoto, Masaya [2 ]
Horibe, Isao [1 ]
Okuno, Yoshiharu [1 ]
机构
[1] Kinki Univ, Dept Appl Chem, Fac Sci & Engn, Higashiosaka, Osaka 5778502, Japan
[2] Milliona Cosmet Co LTD, Higashiosaka, Osaka 5780905, Japan
基金
日本学术振兴会;
关键词
Glomerella cingulata; solid phase microextraction; volatile organic compounds; profile; fungal stage;
D O I
10.5650/jos.57.585
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The profile of volatile organic compounds (VOCs) released from Glomerella cingulata using solid phase microextraction (SPME) with different fibers, Polydimethylsiloxane (PDMS), Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB), Carboxen (TM)/Polydimethylsiloxane (CAR/PDMS) and Divinylbenzene/ Carboxen (TM)/Polydimethylsiloxane (DVB/CAR/PDMS), was investigated. C4-C6 aliphatic alcohols were the predominant fraction of VOCs isolated by CAR/PDMS fiber. Sesquiterpene hydrocarbons represented 20.3% of VOCs isolated by PDMS fiber. During the growth phase, Ochracin was produced in the large majority of VOCs. 3-Methylbutanol and phenylethyl alcohol were found in the log phase of it. Alcohols were found in cultures of higher age, while sesquiterpenes were found to be characteristic of initial growth stage of G. cingulata.
引用
收藏
页码:585 / 590
页数:6
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