Replication protein A modulates the activity of human telomerase in vitro

被引：13

作者：

Rubtsova, M. P. ^{[1
]}

Skvortsov, D. A. ^{[1
]}

Petruseva, I. O. ^{[2
]}

Lavrik, O. I. ^{[2
]}

Spirin, P. V. ^{[3
]}

Prasolov, V. S. ^{[3
]}

Kisseljov, F. L. ^{[4
]}

Dontsova, O. A. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119899, Russia

[2] Russian Acad Sci, Siberian Div, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia

[3] Russian Acad Sci, Inst Mol Biol, Moscow 119991, Russia

[4] Russian Acad Med Sci, Blokhin Canc Res Ctr, Moscow 115478, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2009年 / 74卷 / 01期

关键词：

telomerase; RPA; telomeres; regulation; BINDING PROTEIN; QUADRUPLEX DNA; IMMORTAL CELLS; RPA; COMPLEX; PRIMER; LENGTH; STRAND;

D O I：

10.1134/S0006297909010143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Our aim was to investigate how replication protein A (RPA) in a wide range of concentration can regulate the activity of human telomerase. We used an in vitro system based on human cell extracts with or without RPA. It has been shown that removal of RPA leads to loss of telomerase activity and addition of RPA restores telomerase activity and at the same time promotes telomerase processivity. However, high excess of RPA inhibited telomerase processivity and caused the synthesis of relatively short DNA fragments (about 50-100 nucleotides). We assume that, together with other telomere-binding proteins, RPA may take part in activation of telomere overhang elongation by telomerase at a certain stage of a cell cycle as well as in regulation of telomere length.

引用

页码：92 / 96

页数：5

共 22 条

[1] Functional and dysfunctional roles of quadruplex DNA in cells [J].

Arthanari, H ;

Bolton, PH .

CHEMISTRY & BIOLOGY, 2001, 8 (03) :221-230

[2] Effects of single-stranded DNA binding proteins on primer extension by telomerase [J].

Cohen, S ;

Jacob, E ;

Manor, H .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2004, 1679 (02) :129-140

[3] TELOMERE SHORTENING ASSOCIATED WITH CHROMOSOME INSTABILITY IS ARRESTED IN IMMORTAL CELLS WHICH EXPRESS TELOMERASE ACTIVITY [J].

COUNTER, CM ;

AVILION, AA ;

LEFEUVRE, CE ;

STEWART, NG ;

GREIDER, CW ;

HARLEY, CB ;

BACCHETTI, S .

EMBO JOURNAL, 1992, 11 (05) :1921-1929

[4] A dynamic model for replication protein A (RPA) function in DNA processing pathways [J].

Fanning, Ellen ;

Klimovich, Vitaly ;

Nager, Andrew R. .

NUCLEIC ACIDS RESEARCH, 2006, 34 (15) :4126-4137

[5]

HENRICKSEN LA, 1994, J BIOL CHEM, V269, P11121

[6] Replication protein A (RPA): The eukaryotic SSB [J].

Iftode, C ;

Daniely, Y ;

Borowiec, JA .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 34 (03) :141-180

[7] Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss [J].

Kibe, Tatsuya ;

Ono, Yuuki ;

Sato, Koichiro ;

Ueno, Masaru .

MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (06) :2378-2387

[8] SPECIFIC ASSOCIATION OF HUMAN TELOMERASE ACTIVITY WITH IMMORTAL CELLS AND CANCER [J].

KIM, NW ;

PIATYSZEK, MA ;

PROWSE, KR ;

HARLEY, CB ;

WEST, MD ;

HO, PLC ;

COVIELLO, GM ;

WRIGHT, WE ;

WEINRICH, SL ;

SHAY, JW .

SCIENCE, 1994, 266 (5193) :2011-2015

[9] Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP) [J].

Kim, NW ;

Wu, F .

NUCLEIC ACIDS RESEARCH, 1997, 25 (13) :2595-2597

[10] RPA subunit arrangement near the 3′-end of the primer is modulated by the length of the template strand and cooperative protein interactions [J].

Lavrik, OI ;

Kolpashchikov, DM ;

Weisshart, K ;

Nasheuer, HP ;

Khodyreva, SN ;

Favre, A .

NUCLEIC ACIDS RESEARCH, 1999, 27 (21) :4235-4240

← 1 2 3 →