CRISPR-cas loci profiling of Cronobacter sakazakii pathovars

被引：20

作者：

Ogrodzki, Pauline ^{[1
]}

Forsythe, Stephen James ^{[1
]}

机构：

[1] Nottingham Trent Univ, Sch Sci & Technol, Pathogen Res Grp, Clifton Lane, Nottingham NG11 8NS, England

来源：

FUTURE MICROBIOLOGY | 2016年 / 11卷 / 12期

关键词：

CRISPR-cas; Cronobacter sakazakii; genotyping; MLST; sequence types; INTENSIVE-CARE-UNIT; ENTEROBACTER-SAKAZAKII; NEONATAL MENINGITIS; HORIZONTAL TRANSFER; ESCHERICHIA-COLI; SEQUENCE; BACTERIOPHAGE; EVOLUTIONARY; OUTBREAK; STRAINS;

D O I：

10.2217/fmb-2016-0070

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: Cronobacter sakazakii sequence types 1, 4, 8 and 12 are associated with outbreaks of neonatal meningitis and necrotizing enterocolitis infections. However clonality results in strains which are indistinguishable using conventional methods. This study investigated the use of clustered regularly interspaced short palindromic repeats (CRISPR)-cas loci profiling for epidemiological investigations. Materials & methods: Seventy whole genomes of C. sakazakii strains from four clonal complexes which were widely distributed temporally, geographically and origin of source were profiled. Results & conclusion: All strains encoded the same type I-E subtype CRISPR-cas system with a total of 12 different CRISPR spacer arrays. This study demonstrated the greater discriminatory power of CRISPR spacer array profiling compared with multilocus sequence typing, which will be of use in source attribution during Cronobacter outbreak investigations.

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收藏

页码：1507 / 1519

页数：13

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