Sevoflurane Combined with ATP Activates Caspase-1 and Triggers Caspase-1-Dependent Pyroptosis in Murine J774 Macrophages

被引：22
作者：
Jin, Yue [1 ]
Li, Hui [1 ]
Xie, Guohao [1 ]
Chen, Shuzhen [2 ]
Wu, Shuijin [1 ]
Fang, Xiangming [1 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Anesthesiol, Hangzhou 310003, Zhejiang, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China
来源：
INFLAMMATION | 2013年 / 36卷 / 02期
基金：
国家自然科学基金重大项目; 中国国家自然科学基金;
关键词：
sevoflurane; caspase-1; pyroptosis; ROS; INFLAMMATORY CASPASES; NALP3; INFLAMMASOME; OXIDATIVE STRESS; CARDIOPROTECTION; IL-1-BETA; SECRETION; MECHANISM; PLATFORM;
D O I：
10.1007/s10753-012-9550-6
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Sevoflurane is one of the most commonly used volatile anesthetics. Recent studies have shown that sevoflurane plays an important role in modulation of inflammation and immunity. However, little is known about the related molecular mechanisms. This study was designed to investigate the effects and mechanisms of sevoflurane on inflammatory cell death pyroptosis in the murine macrophage cell line J774 cells. Sevoflurane combined with ATP could increase the level of activated caspase-1, pyroptosis, and reactive oxygen species (ROS). Furthermore, treatment of cells with the caspase-1 inhibitor Ac-YVAD-CMK dramatically decreased the percentage of pyroptosis. In addition, inhibition of ROS with N-acetyl-l-cysteine or diphenyleneiodonium significantly reduced the activated levels of caspase-1. These results demonstrated that sevoflurane combined with ATP could activate caspase-1 and trigger caspase-1-dependent pyroptosis through the modulation of ROS production.
引用
收藏
页码：330 / 336
页数：7
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