IL-1β, IL-10 and TNF-α polymorphisms may affect systemic lupus erythematosus risk and phenotype

ObjectiveSystemic lupus erythematosus (SLE) is an autoimmune disease, and IL-1#, IL-10, and TNF-alpha genes are important in the pathogenesis of this disease. We studied the impact of IL-1#-511, IL-1# +3953, IL-10-592, IL-10-1082, TNF-alpha-308, TNF-alpha-238, and TNF-alpha +489 polymorphisms on SLE risk and phenotype in SLE patients and healthy controls.MethodsWe genotyped SLE patients and healthy controls by real-time PCR on QuantStudio 5 (Applied Biosystems) and measured levels of cytokines by enzyme-linked immunosorbent assay (ELISA).Resultsindicated that TNF-alpha-308, IL-10-592, IL-10-1082, IL-1#-511 and IL-1# +3953 polymorphisms affect SLE risk. Furthermore, we exposed that some of the TNF-alpha +489, TNF-alpha-238, IL-10-1082 and IL-1# +3953 genotypes are connected with the SLE phenotype. Moreover, we discovered the linking between specific genotypes and the serum concentrations of TNF-alpha, IL-1#, and IL-10.ConclusionIn conclusion, our study revealed that IL-1#-511, IL-1# +3953, IL-10-592, IL-10-1082, and TNF-alpha-308 polymorphisms may affect SLE risk and phenotype. words