Epitope mapping of LB509, a monoclonal antibody directed against human α-synuclein

被引：93
作者：
Jakes, R
Crowther, RA
Lee, VMY
Trojanowski, JQ
Iwatsubo, T
Goedert, M
机构：
[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
[2] Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA
[3] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Neuropathol & Neurosci, Tokyo, Japan
来源：
NEUROSCIENCE LETTERS | 1999年 / 269卷 / 01期
关键词：
alpha-synuclein; Parkinson's disease; dementia with Lewy bodies; multiple system atrophy; transgenic mice; epitope mapping;
D O I：
10.1016/S0304-3940(99)00411-5
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
alpha-Synuclein is a 140 amino acid protein that forms the major component of the abnormal filaments that make up the Lewy bod ies and Lewy neu rites of Parkinson's disease a nd dementia with Lewy bodies. It is a Iso the major component of the filamentous glial cytoplasmic inclusions of multiple system atrophy. Here we have used recombinant alpha-synucleins and peptide competition to show that the monoclonal anti-alpha-synuclein antibody LB509 recognizes amino acids 115-122 of human alpha-synuclein. The antibody strongly labelled filaments extracted from multiple system atrophy brain, showing the presence of residues 115-122 of alpha-synuclein. LB509 failed to react with mouse, rat and zebra finch a-synuclein, because of amino acid differences with human alpha-synuclein. Since LB509 recognizes human but not rodent alpha-synuclein, it will be a useful reagent for the characterization of mouse lines transgenic for human a-synuclein. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
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页码：13 / 16
页数：4
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