Liquid chromatographic separation and UV determination of certain anti hypertensive agents

被引：25

作者：

Bhushan, R ^{[1
]}

Gupta, D ^{[1
]}

Singh, SK ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Roorkee 247667, Uttar Pradesh, India

来源：

BIOMEDICAL CHROMATOGRAPHY | 2006年 / 20卷 / 02期

关键词：

amlodipine besylate; enalapril maleate; ramipril; HPLC; TLC; open column chromatography; spectrophotometry; antihypertensive compounds;

D O I：

10.1002/bmc.557

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three antihypertensive agents were extracted and isolated from commercial formulations. These were purified and characterized by melting point, lambda(max). and IR. The percentage recovery by extraction process was in the range 81-91%. Active ingredients from binary formulations were separated by RP-HPLC using methanol-water (50:50 v/v) and by TLC using CHCl3-CH3OH (6:1) as mobile phase. Detection was by UV at 210 nm in HPLC, and by iodine vapors in TLC. The solvent conditions from TLC were transferred to open column chromatographic separation. Quantitative determination was carried out using TLC and column chromatography supplemented with UV spectrophotometry. Recovery was in the range 82-93%. Two combination of drugs, viz. amlodipine + ramipril and amlodipine + enalapril, were separated by the three modes of liquid chromatography. The percentage recovery was in the range 80-92% by open column. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：217 / 224

页数：8

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