THERAPEUTIC EFFECT OF BACCHARIS ANOMALA DC. EXTRACTS ON ACTIVATED HEPATIC STELLATE CELLS

被引：9

作者：

Bassos, Bruno de Souza ^{[1
]}

de Mesquita, Fernanda Cristina ^{[1
,2
]}

Dias, Henrique Bregolin ^{[1
]}

Krause, Gabriele Catyana ^{[1
]}

Scherer, Matheus ^{[1
]}

Santarem, Eliane Romanato ^{[3
]}

de Oliveira, Jarbas Rodrigues ^{[1
]}

机构：

[1] Pontificia Univ Catolica Rio Grande do Sul, Escola Ciencias, Lab Pesquisa Biofis Celular & Inflamacao, Porto Alegre, RS, Brazil

[2] Santos Dumont Inst, Edmond & Lily Safra Int Inst Neurosci, Grad Program Neuroengn, Macaiba, Brazil

[3] Pontificia Univ Catolica Rio Grande do Sul, Escola Ciencias, Lab Biotecnol Vegetal, Porto Alegre, RS, Brazil

来源：

EXCLI JOURNAL | 2019年 / 18卷

关键词：

Hepatic fibrosis; HSCs; Baccharis; PPAR-gamma; phenolic compounds; PHENOLIC-COMPOUNDS; CYCLE ARREST; IN-VIVO; N-ACETYLCYSTEINE; APOPTOSIS; TRIMERA; MECHANISMS; ASTERACEAE; BIOLOGY; LINE;

D O I：

10.17179/excli2018-1696

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The therapeutic potential of Baccharis anomala DC. extracts was evaluated through its cytotoxic and antiproliferative effect and their phenotypic reversion property in activated hepatic stellate cells (HSCs). Baccharis anomala is distributed in Brazil (southeastern and south regions) and used for diuretic effect in folk medicine. Four fractions were obtained from the fractionation of the methanolic extract. Fractions III and IV decreased cell proliferation without increasing cell necrosis markers levels and induced cell cycle arrest in G1 phase. Fraction III induced phenotypic reversion through PPAR-gamma activation pathway, while fraction IV did not alter PPAR-alpha/gamma expression levels, suggesting that there is an independent PPAR-alpha/gamma pathway involved. Hydroxybenzoic, chlorogenic and coumaric acids were identified. Fractions III and IV showed antiproliferative effect and ability to induce reversion of activated phenotype of HSCs.

引用

页码：91 / 105

页数：15

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