SEPARATION OF BETAXOLOL HYDROCHLORIDE WITH NEW BONDED CELLULOSE CHIRAL STATIONARY PHASE AND DETERMINATION OF THE ENANTIOMERS IN PLASMA BY HPLC

被引：4

作者：

Zhou, Jie ^{[1
,2
]}

Liu, Qian ^{[1
]}

Su, Nan ^{[1
]}

Fu, Guangjun ^{[1
]}

Pei, Wenjuan ^{[1
]}

Zhang, Zhenzhong ^{[1
,2
]}

机构：

[1] Zhengzhou Univ, Sch Pharm, Zhengzhou 450001, Henan, Peoples R China

[2] Zhengzhou Univ, Res Ctr Nanotechnol Drug, Zhengzhou 450001, Henan, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2012年 / 35卷 / 13期

关键词：

betaxolol hydrochloride; Chiralpak IB; enantiomeric separation; HPLC; normal phase; plasma samples; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; BETA-BLOCKERS; DRUG; ENANTIOSEPARATION; LEVOBETAXOLOL; URINE; BLOOD;

D O I：

10.1080/10826076.2011.627600

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new liquid chromatographic method was developed for the chiral separation of the enantiomers of betaxolol hydrochloride. The enantiomers were separated by a Chiralpak IB (250 x 4.6 mm, 5 mu m) at 30 degrees C and detected at 274 nm, the mobile phase used was n-hexane-ethanol (95/5, V/V), 0.2% diethylamine (DEA), with a flow rate of 0.8mL . min(-1). The enantiomers of betaxolol hydrochloride were separated for the first time with the Chiralpak IB column, and the resolution was 5.26. With the fluorescence detection (lambda(ex) = 227 nm, lambda(em) = 305 nm), the method was applicable for the determination of the enantiomers of betaxolol hydrochloride in rabbit plasma.

引用

页码：1767 / 1778

页数：12

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