SEPARATION OF BETAXOLOL HYDROCHLORIDE WITH NEW BONDED CELLULOSE CHIRAL STATIONARY PHASE AND DETERMINATION OF THE ENANTIOMERS IN PLASMA BY HPLC

被引：4

作者：

Zhou, Jie ^{[1
,2
]}

Liu, Qian ^{[1
]}

Su, Nan ^{[1
]}

Fu, Guangjun ^{[1
]}

Pei, Wenjuan ^{[1
]}

Zhang, Zhenzhong ^{[1
,2
]}

机构：

[1] Zhengzhou Univ, Sch Pharm, Zhengzhou 450001, Henan, Peoples R China

[2] Zhengzhou Univ, Res Ctr Nanotechnol Drug, Zhengzhou 450001, Henan, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2012年 / 35卷 / 13期

关键词：

betaxolol hydrochloride; Chiralpak IB; enantiomeric separation; HPLC; normal phase; plasma samples; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; BETA-BLOCKERS; DRUG; ENANTIOSEPARATION; LEVOBETAXOLOL; URINE; BLOOD;

D O I：

10.1080/10826076.2011.627600

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new liquid chromatographic method was developed for the chiral separation of the enantiomers of betaxolol hydrochloride. The enantiomers were separated by a Chiralpak IB (250 x 4.6 mm, 5 mu m) at 30 degrees C and detected at 274 nm, the mobile phase used was n-hexane-ethanol (95/5, V/V), 0.2% diethylamine (DEA), with a flow rate of 0.8mL . min(-1). The enantiomers of betaxolol hydrochloride were separated for the first time with the Chiralpak IB column, and the resolution was 5.26. With the fluorescence detection (lambda(ex) = 227 nm, lambda(em) = 305 nm), the method was applicable for the determination of the enantiomers of betaxolol hydrochloride in rabbit plasma.

引用

页码：1767 / 1778

页数：12

共 19 条

[1] Bono G. D., 1995, SYNTHESIS-STUTTGART, V6, P699
[2] DETERMINATION OF BETAXOLOL IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
CANAL, M
FLOUVAT, B
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 342 (01): : 212 - 215
[3] SIMPLE METHOD FOR ROUTINE DETERMINATION OF BETAXOLOL IN BLOOD AND URINE BY AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORIMETRIC DETECTION
CAQUERET, H
BIANCHETTI, G
[J]. JOURNAL OF CHROMATOGRAPHY, 1984, 311 (01): : 199 - 205
[4] Direct liquid chromatographic enantioseparation of sotalol and other beta-blockers using an alpha(1)-acid glycoprotein-based chiral stationary phase
Ceccato, A
Hubert, P
Crommen, J
[J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 760 (02) : 193 - 203
[5] Determination of betaxolol in human aqueous humour by high-performance liquid chromatography with fluorescence detection
Dulger, B
Basci, NE
Sagdic-Yalvac, I
Temizer, A
[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 772 (01): : 179 - 183
[6] CHIRAL SEPARATIONS OF BETA-BLOCKING DRUG SUBSTANCES USING CHIRAL STATIONARY PHASES
EKELUND, J
VANARKENS, A
BRONNUMHANSEN, K
FICH, K
OLSEN, L
PETERSEN, PV
[J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 708 (02) : 253 - 261
[7] Hefnawy MM, 2006, ANAL CHEM INSIGHTS, V1, P13
[8] DIRECT ENANTIOMERIC SEPARATION OF BETAXOLOL WITH APPLICATIONS TO ANALYSIS OF BULK DRUG AND BIOLOGICAL SAMPLES
KRSTULOVIC, AM
FOUCHET, MH
BURKE, JT
GILLET, G
DURAND, A
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 452 : 477 - 483
[9] Direct Resolution of the Enantiomers of Betaxolol and Related Intermediates, and Its Application
Li, Yong-Hong
Huang, Li-Hua
Zhang, Shu-Sheng
Liu, Hong-Min
[J]. CHROMATOGRAPHIA, 2010, 71 (11-12) : 987 - 991
[10] Effectiveness of levobetaxolol and timolol at blunting retinal ischaemia is related to their calcium and sodium blocking activities: relevance to glaucoma
Osborne, NN
Wood, JPM
Chidlow, G
Casson, R
DeSantis, L
Schmidt, KG
[J]. BRAIN RESEARCH BULLETIN, 2004, 62 (06) : 525 - 528

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