SEPARATION OF BETAXOLOL HYDROCHLORIDE WITH NEW BONDED CELLULOSE CHIRAL STATIONARY PHASE AND DETERMINATION OF THE ENANTIOMERS IN PLASMA BY HPLC

被引：4
|
作者：
Zhou, Jie [1 ,2 ]
Liu, Qian [1 ]
Su, Nan [1 ]
Fu, Guangjun [1 ]
Pei, Wenjuan [1 ]
Zhang, Zhenzhong [1 ,2 ]
机构：
[1] Zhengzhou Univ, Sch Pharm, Zhengzhou 450001, Henan, Peoples R China
[2] Zhengzhou Univ, Res Ctr Nanotechnol Drug, Zhengzhou 450001, Henan, Peoples R China
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2012年 / 35卷 / 13期
关键词：
betaxolol hydrochloride; Chiralpak IB; enantiomeric separation; HPLC; normal phase; plasma samples; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; BETA-BLOCKERS; DRUG; ENANTIOSEPARATION; LEVOBETAXOLOL; URINE; BLOOD;
D O I：
10.1080/10826076.2011.627600
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new liquid chromatographic method was developed for the chiral separation of the enantiomers of betaxolol hydrochloride. The enantiomers were separated by a Chiralpak IB (250 x 4.6 mm, 5 mu m) at 30 degrees C and detected at 274 nm, the mobile phase used was n-hexane-ethanol (95/5, V/V), 0.2% diethylamine (DEA), with a flow rate of 0.8mL . min(-1). The enantiomers of betaxolol hydrochloride were separated for the first time with the Chiralpak IB column, and the resolution was 5.26. With the fluorescence detection (lambda(ex) = 227 nm, lambda(em) = 305 nm), the method was applicable for the determination of the enantiomers of betaxolol hydrochloride in rabbit plasma.
引用
收藏
页码：1767 / 1778
页数：12
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