Seroprevalence and isolation of Toxoplasma gondii from free-range chickens from Espirito Santo state, southeastern Brazil

被引：35

作者：

Beltrame, M. A. V. ^{[2
]}

Pena, H. F. J. ^{[3
]}

Ton, N. C. ^{[2
]}

Lino, A. J. B. ^{[2
]}

Gennari, S. M. ^{[3
]}

Dubey, J. P. ^{[4
]}

Pereira, F. E. L. ^{[1
]}

机构：

[1] Univ Fed Espirito Santo, BR-29040091 Vitoria, ES, Brazil

[2] Univ Vila Velha, Curso Med Vet, BR-29102690 Vila Velha, ES, Brazil

[3] Univ Sao Paulo, Dept Med Vet Preventiva & Saude Anim, Fac Med Vet & Zootecn, BR-05508270 Sao Paulo, Brazil

[4] USDA ARS, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA

来源：

VETERINARY PARASITOLOGY | 2012年 / 188卷 / 3-4期

关键词：

Toxoplasma gondii; Chickens; Gallus domesticus; Seroprevalence; Isolation; Espirito Santo state; Brazil; SAO-PAULO STATE; PREVALENCE; RESPONSES;

D O I：

10.1016/j.vetpar.2012.03.053

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Prevalence of Toxoplasma gondii infection in 510 free-range (FR) chickens (380 from 33 small farms, and 130 from a slaughter house for FR chickens) from Espirito Santo state, southeastern Brazil, was investigated. Antibodies to T. gondii were sought using commercial indirect haemagglutination (IHAT, Imuno-HAI Toxo (R), Wama Diagnostica, Sao Paulo, Brazil, cut-off 1:16) and the modified agglutination test (MAT, cut-off 1:25) tests. Attempts were made to isolate viable T. gondii from seropositive chickens by bioassay in mice. Pooled samples of brain, heart and quadriceps muscle of one thigh (total 40 g) from 64 chickens with IHAT titers of >= 1:16 were minced, digested in pepsin and bioassayed in mice. Antibodies to T. gondii were found in 40.4% (206/510) FR chickens by IHAT (titer >= 1:16) and 38.8% (198/510) by MAT (titer >= 1:25); concordance between IHAT and MAT was 81.6% (kappa index = 0.614). Viable T. gondii was isolated (designated TgCkBr234-281) from 48 of 64(75%) seropositive (IHAT titers >= 1:32) FR chickens. Most isolates of T. gondii were virulent for mice; 100% of mice inoculated with 44 of 48 isolates died of toxoplasmosis within 30 days post inoculation (p.i). An epidemiological investigation revealed that people living in rural areas have little knowledge about the parasite and about the risk of acquiring it from raw meat. Results indicated that the locally available IHAT was useful for screening of chicken sera for T. gondii antibodies. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：225 / 230

页数：6

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