Cellular entry and nuclear targeting by a highly anionic molecular umbrella

被引：19

作者：

Mehiri, Mohamed ^{[1
]}

Jing, Bingwen ^{[1
]}

Ringhoff, Danielle ^{[1
]}

Janout, Vaclav ^{[1
]}

Cassimeris, Lynne ^{[2
]}

Regen, Steven L. ^{[1
]}

机构：

[1] Lehigh Univ, Dept Chem, Bethlehem, PA 18015 USA

[2] Lehigh Univ, Dept Biol Sci, Bethlehem, PA 18015 USA

来源：

BIOCONJUGATE CHEMISTRY | 2008年 / 19卷 / 08期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1021/bc8001826

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fluorescently labeled, persulfated molecular umbrella (1) has been synthesized from cholic acid, lysine, spermine, and Coumarin 343 and found capable of entering live HeLa cells. The distributions of 1 throughout the cytoplasm and the nucleus were diffuse and punctate, respectively. This finding, together with its ability to cross liposomal membranes by passive diffusion, suggests that passive diffusion plays a significant role in the ability of I to enter cells. The fact that I is concentrated at the nucleus raises the possibility that molecular umbrellas of this type could be used for the nuclear targeting of drugs.

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收藏

页码：1510 / 1513

页数：4

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