Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls

被引：246

作者：

Barker, Nick ^{[3
]}

van Oudenaarden, Alexander ^{[1
,2
,4
,5
]}

Clevers, Hans ^{[1
,2
]}

机构：

[1] Royal Netherlands Acad Arts & Sci, Hubrecht Inst, NL-3584 CT Utrecht, Netherlands

[2] Univ Med Ctr Utrecht, NL-3584 CT Utrecht, Netherlands

[3] Inst Med Biol, Immunos 138648, Singapore

[4] MIT, Dept Phys, Cambridge, MA 02139 USA

[5] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

CELL STEM CELL | 2012年 / 11卷 / 04期

关键词：

WNT/BETA-CATENIN; SELF-RENEWAL; WNT RECEPTORS; IN-VITRO; LGR5; EPITHELIUM; IDENTIFICATION; MARKER; COLON; PROGENITORS;

D O I：

10.1016/j.stem.2012.09.009

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Decades ago, two nonoverlapping crypt stem cell populations were proposed: Leblond's Crypt Base Columnar (CBC) cell and Potten's +4 cell. The identification of CBC markers including Lgr5 has confirmed Leblond's predictions that CBC cells are anatomically distinct, long-lived stem cells that permanently cycle. While Potten originally described +4 cells as proliferative and unusually radiation-sensitive, recent efforts to identify +4 stem cells have focused on the identification of cells that are quiescent and radiation-resistant. Here, we describe commonalities and discrepancies between the individual studies and discuss challenges of marker-based lineage tracing.

引用

页码：452 / 460

页数：9

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