13C SPE MAS measurement of ligand concentration in compressible chromatographic beads

被引：3
作者：
Elwinger, Fredrik [1 ,2 ]
Dvinskikh, Sergey V. [1 ]
Furo, Istvan [1 ]
机构：
[1] KTH Royal Inst Technol, Div Appl Phys Chem, SE-10044 Stockholm, Sweden
[2] GE Healthcare Biosci AB, SE-75184 Uppsala, Sweden
来源：
MAGNETIC RESONANCE IN CHEMISTRY | 2015年 / 53卷 / 08期
基金：
瑞典研究理事会;
关键词：
NMR; C-13; solid-state NMR; quantitative NMR; agarose; pore; SOLID-STATE NMR; NUCLEAR-MAGNETIC-RESONANCE; ANGLE-SPINNING NMR; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; SUPERCRITICAL CARBON-DIOXIDE; MODEL ORGANIC-COMPOUNDS; STATIONARY PHASES; QUANTITATIVE-DETERMINATION; CROSS-POLARIZATION; LIQUID-CHROMATOGRAPHY;
D O I：
10.1002/mrc.4257
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, C-13 single pulse excitation magic angle spinning NMR experiment with broadband H-1 decoupling is used to determine the peak integrals for a butyl ligand in the spectrum of a dried chromatography medium. Within a carefully controlled protocol, those integrals compared with that of the internal reference compound dimethyl sulfone provide the required volume concentration with an accuracy of ca 2%. The effects of temperature, degree of hydration, and other experimental parameters are discussed. Copyright (C) 2015 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.
引用
收藏
页码：572 / 577
页数：6
相关论文
共 50 条
  • [21] Triple Resonance Cross-Polarization for More Sensitive 13C MAS NMR Spectroscopy of Deuterated Proteins
    Akbey, Uemit
    Camponeschi, Francesca
    van Rossum, Barth-Jan
    Oschkinat, Hartmut
    CHEMPHYSCHEM, 2011, 12 (11) : 2092 - 2096
  • [22] Structural Characterization of Hydrothermal Carbon Spheres by Advanced Solid-State MAS 13C NMR Investigations
    Baccile, Niki
    Laurent, Guillaume
    Babonneau, Florence
    Fayon, Franck
    Titirici, Maria-Magdalena
    Antonietti, Markus
    JOURNAL OF PHYSICAL CHEMISTRY C, 2009, 113 (22) : 9644 - 9654
  • [23] Spectral editing in 13C MAS NMR under moderately fast spinning conditions
    De Vita, E
    Frydman, L
    JOURNAL OF MAGNETIC RESONANCE, 2001, 148 (02) : 327 - 337
  • [24] 13C cross-polarization MAS NMR study of some steroidal sapogenins
    Wawer, I
    Nartowska, J
    Cichowlas, AA
    SOLID STATE NUCLEAR MAGNETIC RESONANCE, 2001, 20 (1-2) : 35 - 45
  • [25] Some triple resonance experiments in solid-state CP MAS NMR:: 51V/29Si, 31P/13C, and 29Si/13C
    Bonhomme, C
    Coelho, C
    Azaïs, T
    Bonhomme-Coury, L
    Babonneau, F
    Maquet, J
    Thouvenot, R
    COMPTES RENDUS CHIMIE, 2006, 9 (3-4) : 466 - 471
  • [26] Solid-state CP/MAS 13C NMR studies on conformational polymorphism in sertraline hydrochloride, an antidepressant drug
    Novoselsky, A
    Glaser, R
    MAGNETIC RESONANCE IN CHEMISTRY, 2002, 40 (11) : 723 - 728
  • [27] X-ray and 13C CP MAS investigations of structure of two genistein derivatives
    Grynkiewicz, G
    Zegrocka-Stendel, O
    Pucko, W
    Ramza, J
    Koscielecka, A
    Kolodziejski, W
    Wozniak, K
    JOURNAL OF MOLECULAR STRUCTURE, 2004, 694 (1-3) : 121 - 129
  • [28] SOMnmR: An R Package for Analyzing CP MAS 13C NMR Spectra of Environmental Samples
    Hurtarte, Luis Carlos Colocho
    Garcia-Franco, Noelia
    Ayala, Gabriela Villalba
    Prietzel, Joerg
    Koegel-Knabner, Ingrid
    JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES, 2025, 130 (02)
  • [29] A CP/MAS 13C NMR investigation of cellulose ultrastructure in traditional Chinese handmade papers
    Liu, Peng
    Jin, Chao
    Zhang, Kai
    Xue, Yu
    Gao, Boxu
    Jia, Yingshuai
    Yan, Yueer
    Zhang, Hongbin
    Wang, Sinong
    Tang, Yi
    HOLZFORSCHUNG, 2022, 76 (09) : 853 - 862
  • [30] Structural characterization of Huadian oil shale kerogen by using 13C DP/MAS NMR
    Wang, Xiaoye
    You, Yulong
    Mu, Mao
    Han, Xiangxin
    Shu, Jie
    Jiang, Xiumin
    OIL SHALE, 2021, 38 (03) : 181 - 198
12345