13C SPE MAS measurement of ligand concentration in compressible chromatographic beads

被引：3

作者：

Elwinger, Fredrik ^{[1
,2
]}

Dvinskikh, Sergey V. ^{[1
]}

Furo, Istvan ^{[1
]}

机构：

[1] KTH Royal Inst Technol, Div Appl Phys Chem, SE-10044 Stockholm, Sweden

[2] GE Healthcare Biosci AB, SE-75184 Uppsala, Sweden

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2015年 / 53卷 / 08期

基金：

瑞典研究理事会;

关键词：

NMR; C-13; solid-state NMR; quantitative NMR; agarose; pore; SOLID-STATE NMR; NUCLEAR-MAGNETIC-RESONANCE; ANGLE-SPINNING NMR; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; SUPERCRITICAL CARBON-DIOXIDE; MODEL ORGANIC-COMPOUNDS; STATIONARY PHASES; QUANTITATIVE-DETERMINATION; CROSS-POLARIZATION; LIQUID-CHROMATOGRAPHY;

D O I：

10.1002/mrc.4257

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, C-13 single pulse excitation magic angle spinning NMR experiment with broadband H-1 decoupling is used to determine the peak integrals for a butyl ligand in the spectrum of a dried chromatography medium. Within a carefully controlled protocol, those integrals compared with that of the internal reference compound dimethyl sulfone provide the required volume concentration with an accuracy of ca 2%. The effects of temperature, degree of hydration, and other experimental parameters are discussed. Copyright (C) 2015 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.

引用

页码：572 / 577

页数：6

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