Correction for specimen movement and rotation errors for in-vivo Optical Projection Tomography

被引：39

作者：

Birk, Udo Jochen ^{[1
,2
,3
]}

Rieckher, Matthias ^{[4
]}

Konstantinides, Nikos ^{[4
]}

Darrell, Alex ^{[5
]}

Sarasa-Renedo, Ana ^{[4
]}

Meyer, Heiko ^{[1
]}

Tavernarakis, Nektarios ^{[4
]}

Ripoll, Jorge ^{[1
]}

机构：

[1] Fdn Res & Technol Hellas FORTH, Inst Elect Struct & Laser, Iraklion 71110, Greece

[2] Univ Heidelberg, Kirchhoff Inst Phys, INF 227, D-69120 Heidelberg, Germany

[3] Med Laserzentrum Lubeck GmbH, D-23452 Lubeck, Germany

[4] FORTH, Inst Mol Biol & Biotechnol, Iraklion 71110, Greece

[5] FORTH, Inst Comp Sci, Iraklion 71110, Greece

来源：

BIOMEDICAL OPTICS EXPRESS | 2010年 / 1卷 / 01期

关键词：

D O I：

10.1364/BOE.1.000087

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of optical projection tomography to in-vivo experiments is limited by specimen movement during the acquisition. We present a set of mathematical correction methods applied to the acquired data stacks to correct for movement in both directions of the image plane. These methods have been applied to correct experimental data taken from in-vivo optical projection tomography experiments in Caenorhabditis elegans. Successful reconstructions for both fluorescence and white light (absorption) measurements are shown. Since no difference between movement of the animal and movement of the rotation axis is made, this approach at the same time removes artifacts due to mechanical drifts and errors in the assumed center of rotation. (C) 2010 Optical Society of America

引用

页码：87 / 96

页数：10

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