Effects of NG-monomethyl-L-arginine (NMMA) and catalase on intracellular survival of Vibrio alginolyticus in macrophages of large yellow croaker Pseudosciaena crocea

The intracellular survival of Vibrio alginolyticus and Vibrio parahaemolyticus in large yellow croaker macrophages of Pseudosciaena crocea was investigated. In addition, the effects of N-G-monomethyl-L-arginine (NMMA) and catalase on the interaction of V. alginolyticus with macrophages from head kidney and on macrophages reactive nitrogen intermediate (RNI) and reactive oxygen intermediate (ROI) in vitro were determined. V. alginolyticus ND-01 was able to survive in macrophages from head kidney of large yellow croakers for at least 3 h, while V. paraheamolyticus 1.1614 could not survive in the macrophages for 1 h. Intracellular bacterial survival was affected by the addition of specific inhibitors of macrophage oxidative function. Exposure of macrophages to NMMA and catalase decreased the number of viable cells of V. alginolyticus inside large yellow croaker macrophages. Furthermore, a close correlation was observed between the number of intracellular survival bacteria with the amount of NO and H2O2 produced by macrophages.