Genetic variability and potential recombination events in the HC-Pro gene of sugarcane streak mosaic virus

被引：22

作者：

Bagyalakshmi, K. ^{[1
]}

Parameswari, B. ^{[2
]}

Chinnaraja, C. ^{[1
]}

Karuppaiah, R. ^{[1
]}

Kumar, V. Ganesh ^{[1
]}

Viswanathan, R. ^{[1
]}

机构：

[1] ICAR Res Complex, Sugarcane Breeding Inst, Div Crop Protect, Plant Pathol Sect, Coimbatore 641007, Tamil Nadu, India

[2] Sugarcane Breeding Inst Reg Ctr, Karnal 132001, Haryana, India

来源：

ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 07期

关键词：

DIVERSITY; EVOLUTION; POTATO;

D O I：

10.1007/s00705-012-1297-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sugarcane streak mosaic virus (SCSMV), a member of the family Potyviridae, is an important viral pathogen affecting sugarcane production in India. The variability in the nucleotide (nt) and amino acid (aa) sequences of helper component proteinase (HC-Pro) of SCSMV isolates from India was investigated and compared with those of previously published virus isolates from different Asian countries. Comparison of all of the sequenced virus isolates revealed a high level of diversity in the HC-Pro gene (72-97% nt sequence identity; 83-99% aa sequence identity), and the Indian isolates were found to be the most divergent (up to 12% variation at the amino acid level). Phylogenetic analysis revealed clustering of 16 SCSMV isolates into two groups. Group I included isolates from India and Pakistan, and group II consisted of isolates from Japan and Indonesia. Recombination analysis revealed nine potentially significant recombination events, and putative recombination sites were identified throughout the HC-Pro gene. Analysis of selection pressure indicated that the HC-Pro gene of SCSMV is under strong negative selection. It is likely that recombination, along with strong negative selection, enhances the speed of elimination of deleterious mutations in the HC-Pro gene.

引用

页码：1371 / 1375

页数：5

共 23 条

[1] A virus reveals population structure and recent demographic history of its carnivore host [J].

Biek, R ;

Drummond, AJ ;

Poss, M .

SCIENCE, 2006, 311 (5760) :538-541

[2]

Chatenet M., 2005, P INT SOC SUG CAN TE, V25, P656

[3] First occurrence of Sugarcane streak mosaic virus infecting sugarcane in Indonesia [J].

Damayanti, Tri Asmira ;

Putra, Lilik Koesmihartono .

JOURNAL OF GENERAL PLANT PATHOLOGY, 2011, 77 (01) :72-74

[4] Datamonkey 2010: a suite of phylogenetic analysis tools for evolutionary biology [J].

Delport, Wayne ;

Poon, Art F. Y. ;

Frost, Simon D. W. ;

Pond, Sergei L. Kosakovsky .

BIOINFORMATICS, 2010, 26 (19) :2455-2457

[5] Variability and genetic structure of plant virus populations [J].

García-Arenal, F ;

Fraile, A ;

Malpica, JM .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 2001, 39 :157-186

[6]

Gaur R. K., 2006, Sugar Cane International, V24, P13

[7] Molecular cloning, sequencing, and phylogenetic relationships of a new potyvirus: Sugarcane streak mosaic virus, and a reevaluation of the classification of the Potyviridae [J].

Hall, JS ;

Adams, B ;

Parsons, TJ ;

French, R ;

Lane, LC ;

Jensen, SG .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 1998, 10 (03) :323-332

[8] Taxonomic position of sugarcane streak mosaic virus in the family Potyvitidae [J].

Hema, M ;

Sreenivasulu, P ;

Savithri, HS .

ARCHIVES OF VIROLOGY, 2002, 147 (10) :1997-2007

[9] Molecular characterization and interviral relationships of a flexuous filamentous virus causing mosaic disease of sugarcane (Saccharum officinarum L.) in India [J].

Hema, M ;

Joseph, J ;

Gopinath, K ;

Sreenivasulu, P ;

Savithri, HS .

ARCHIVES OF VIROLOGY, 1999, 144 (03) :479-490

[10] DELETERIOUS MUTATIONS AND THE EVOLUTION OF SEXUAL REPRODUCTION [J].

KONDRASHOV, AS .

NATURE, 1988, 336 (6198) :435-440

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