Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Cote d'Ivoire: occurrence and evidence for environmental contamination

被引：60
作者：
Berrilli, Federica [1 ]
D'Alfonso, Rossella [2 ,3 ]
Giangaspero, Annunziata [4 ]
Marangi, Marianna [4 ]
Randonisio, Olga [5 ]
Kabore, Yolande [3 ]
Gle, Christoph [3 ]
Cianfanelli, Cristina [1 ]
Lauro, Renato [2 ]
Di Cave, David [1 ]
机构：
[1] Univ Roma Tor Vergata, Dept Publ Hlth & Cell Biol, I-00133 Rome, Italy
[2] Univ Roma Tor Vergata, Dept Internal Med, I-00133 Rome, Italy
[3] Ctr Don Orione Handicapes Phys Bonoua, Bonoua, Cote Ivoire
[4] Univ Foggia, Dept PrIME, I-71100 Foggia, Italy
[5] Univ Bari, Dept Internal Med Immunol & Infect Dis, I-70124 Bari, Italy
来源：
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2012年 / 106卷 / 03期
关键词：
Giardia duodenalis; Cryptosporidium spp; Molecular characterization; Environmental contamination; Zoonosis; Cote d'Ivoire; MOLECULAR CHARACTERIZATION; PARVUM OOCYSTS; PREVALENCE; LAMBLIA; BIRDS; DOGS; SCHOOLCHILDREN; EPIDEMIOLOGY; INTESTINALIS; TRANSMISSION;
D O I：
10.1016/j.trstmh.2011.12.005
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Giardia duodenalis genotypes and Cryptosporidium species were studied in humans and free-ranging animals living in closed enclaves in Cote d'Ivoire. Three hundred and seven stool samples were tested from humans, and 47 from freely roaming domestic animals (dogs, goats, ducks, chickens). Molecular characterization of the isolates was performed by sequence analysis of a portion of the SSU-rDNA for Giardia and the COWP gene for Cryptosporidium, and a beta-giardin SYBR-green real-time PCR was also used to confirm the assignment of Giardia isolates to Assemblages. In humans, genotyping of Giardia assigned many of the sequences (43/56 by the SSU-rDNA gene, and 36/61 by the beta-giardin gene) to Assemblage B. The animal species harboured only zoonotic Assemblages A and B, except for dogs, in which host specific Assemblages C and D were also detected. Cryptosporidium meleagridis, C. parvum and C. hominis were detected in humans. while among the animals only chickens were found positive for oocysts, identified as C. meleagridis and C. parvum. The results provide further evidence about the role of free-ranging domestic animals living closely with humans in the environmental dissemination and potential transmission of these anthropozoonotic pathogens to humans. (C) 2011 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
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页码：191 / 195
页数：5
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