Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination
The mechanical properties of the different germ layers of the early mammalian embryo are likely to be critical for morphogenesis. Cytoskeleton components (actin and myosin, microtubules, intermediate filaments) are major determinants of epithelial plasticity and resilience to stress. Here, we take advantage of a mouse reporter for Keratin 8 to record the pattern of the keratin intermediate filaments network in the first epithelia of the developing mouse embryo. At the blastocyst stage, Keratin 8 is strongly expressed in the trophectoderm, and undetectable in the inner cell mass and its derivatives, the epiblast and primitive endoderm. Visceral endoderm cells that differentiate from the primitive endoderm at the egg cylinder stage display apical Keratin 8 filaments. Upon migration of the Anterior Visceral Endoderm and determination of the anterior-posterior axis, Keratin 8 becomes regionally distributed, with a stronger expression in embryonic, compared to extra-embryonic, visceral endoderm. This pattern emerges concomitantly to a modification of the distribution of Filamentous (F)-actin, from a cortical ring to a dense apical shroud, in extra-embryonic visceral endoderm only. Those regional characteristics are maintained across gastrulation. Interestingly, for each stage and region of the embryo, adjacent germ layers display contrasted levels of keratin filaments, which may play a role in their adaptation to growth and morphological changes.
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY USASloan Kettering Inst, Dev Biol Program, New York, NY USA
Ferrer-Vaquer, Anna
Viotti, Manuel
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Sloan Kettering Inst, Dev Biol Program, New York, NY USA
Cornell Univ, Weill Grad Sch Med Sci, Biochem Cell & Mol Biol Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY USA
Viotti, Manuel
Hadjantonakis, Anna-Katerina
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机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY USASloan Kettering Inst, Dev Biol Program, New York, NY USA
机构:
Mem Sloan Kettering Canc Ctr, Sloan Kettering Inst, Dev Biol Program, 1275 York Ave, New York, NY 10021 USAUniv Copenhagen, Novo Nordisk Fdn, Ctr Stem Cell Biol, DK-2200 Copenhagen, Denmark
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Cornell Univ, Weill Grad Sch Med Sci, Neurosci Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Kwon, Gloria S.
Viotti, Manuel
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h-index: 0
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Cornell Univ, Weill Grad Sch Med Sci, Cell & Mol Biol Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Viotti, Manuel
Hadjantonakis, Anna-Katerina
论文数: 0引用数: 0
h-index: 0
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY USASloan Kettering Inst, Dev Biol Program, New York, NY USA
Ferrer-Vaquer, Anna
Viotti, Manuel
论文数: 0引用数: 0
h-index: 0
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY USA
Cornell Univ, Weill Grad Sch Med Sci, Biochem Cell & Mol Biol Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY USA
Viotti, Manuel
Hadjantonakis, Anna-Katerina
论文数: 0引用数: 0
h-index: 0
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY USASloan Kettering Inst, Dev Biol Program, New York, NY USA
机构:
Mem Sloan Kettering Canc Ctr, Sloan Kettering Inst, Dev Biol Program, 1275 York Ave, New York, NY 10021 USAUniv Copenhagen, Novo Nordisk Fdn, Ctr Stem Cell Biol, DK-2200 Copenhagen, Denmark
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Cornell Univ, Weill Grad Sch Med Sci, Neurosci Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Kwon, Gloria S.
Viotti, Manuel
论文数: 0引用数: 0
h-index: 0
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Cornell Univ, Weill Grad Sch Med Sci, Cell & Mol Biol Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA
Viotti, Manuel
Hadjantonakis, Anna-Katerina
论文数: 0引用数: 0
h-index: 0
机构:
Sloan Kettering Inst, Dev Biol Program, New York, NY 10021 USASloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA