Separation of antibacterial biocompounds from Hancornia speciosa leaves by a sequential process of pressurized liquid extraction

被引：24

作者：

Barbosa, Andriele M. ^{[1
,2
,3
]}

Santos, Klebson S. ^{[1
]}

Borges, Gustavo R. ^{[1
,2
,3
]}

Muniz, Ana V. C. S. ^{[4
]}

Mendonca, Flavia M. R. ^{[1
,2
,3
]}

Pinheiro, Malone S. ^{[2
,3
]}

Franceschi, Elton ^{[1
,2
,3
]}

Dariva, Claudio ^{[1
,2
,3
]}

Padilha, Francine F. ^{[1
,2
,3
]}

机构：

[1] ITP, Ctr Study Colloidal Syst NUESC, Av Murilo Dantas 300, BR-49032490 Aracaju, SE, Brazil

[2] Tiradentes Univ UNIT, Postgrad Programme Proc Engn PEP, Hlth & Environm PSA, Av Murilo Dantas 300, BR-49032490 Aracaju, SE, Brazil

[3] PBI, Ind Biotechnol, Av Murilo Dantas 300, BR-49032490 Aracaju, SE, Brazil

[4] Embrapa Coastal Tablelands, Av Beira Mar 3-250, BR-49025040 Aracaju, SE, Brazil

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2019年 / 222卷

关键词：

Hancornia speciosa; Phenolic compounds; Pressurized liquid extraction; Antioxidant activity; Antibacterial activity; Multidrug-resistance; PHENOLIC-COMPOUNDS; BIOACTIVE COMPOUNDS; SUPERCRITICAL-FLUID; ANTIOXIDANT; FLAVONOIDS; RECOVERY; OIL; L; PHYTOCHEMICALS; SUSCEPTIBILITY;

D O I：

10.1016/j.seppur.2019.04.022

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Hancornia speciosa has bioactive molecules such as phenolic compounds which exhibit antioxidant and antimicrobial properties. Nevertheless, the separation of these substances from a medicinal plant can be different according to the extraction process and solvent used. Thus, the present study aimed was to separate phenolic compounds from Hancornia speciosa leaves in three steps by using a sequential pressurized liquid extraction and evaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to 60 degrees C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 +/- 0.2%) showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 degrees C exhibited the highest amount of total phenolic compounds (347.0 +/- 4.12 mg/g extract). Fractionated ethyl acetate extract (FAE) at 60 degrees C presented improved quantity of total flavonoids (230.4 +/- 3.5 mg/g extract) and rutin content (16.6 +/- 0.3 mg/mL extract). The antioxidant activity results for IC50 of DPPH indicated that FEE (3.8 +/- 0.1 mu g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of multidrug-resistant E. coli.

引用

页码：390 / 395

页数：6

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