Designer cells for stereocomplementary de novo enzymatic cascade reactions based on laboratory evolution

被引：45
作者：
Agudo, Ruben [1 ,2 ]
Reetz, Manfred T. [1 ,2 ]
机构：
[1] Univ Marburg, Fachbereich Chem, D-35032 Marburg, Germany
[2] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 93期
关键词：
CYTOCHROME P450BM-3; DIRECTED EVOLUTION; NEXT-GENERATION; BIOCATALYSTS; REDUCTION; MONOOXYGENASE; MUTAGENESIS; OXIDATION; CYP102A1; REVEALS;
D O I：
10.1039/c3cc46229c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Designer cells for a synthetic cascade reaction harnessing selective redox reactions were devised, featuring two successive regioselective P450-catalyzed CH-activating oxidations of 1-cyclohexene carboxylic acid methyl ester followed by stereoselective olefin-reduction catalysed by (R)- or (S)-selective mutants of an enoate reductase.
引用
收藏
页码：10914 / 10916
页数：3
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