Reconstruction of complementary images in second harmonic generation microscopy

被引：21

作者：

Gao, Liang ^{[1
]}

Jin, Lei

Xue, Ping

Xu, Jun

Wang, Yi

Ma, Hui

Chen, Dieyan

机构：

[1] Tsinghua Univ, Dept Phys, Lab Atom Mol & Nanosci, Beijing 100084, Peoples R China

[2] Tsinghua Univ, Dept Chem Engn, Beijing 100084, Peoples R China

来源：

OPTICS EXPRESS | 2006年 / 14卷 / 11期

关键词：

D O I：

10.1364/OE.14.004727

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Second harmonic generation microscopy(SHGM) has become widely used to image biological samples. Due to the complexity of biological samples, more and more effort has been put on polarization imaging in SHGM technology to uncover their structures. In this work, we put forward a novel stitching method based on careful mathematical calculation, and accomplish it by rotating laser polarization. We first show its validity in imaging a perfectly synthesized bio-origin polymer poly (3-hyroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Then, we test its power by getting a true image of fibrillar collagen structure of rat-tail tendon. (c) 2006 Optical Society of America.

引用

页码：4727 / 4735

页数：9

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