Transcriptional and Microscopic Analyses of Citrus Stem and Root Responses to Candidatus Liberibacter asiaticus Infection

被引:122
作者
Aritua, Valente [1 ]
Achor, Diann [2 ]
Gmitter, Frederick G. [3 ]
Albrigo, Gene [3 ]
Wang, Nian [1 ]
机构
[1] Univ Florida, Dept Microbiol & Cell Sci, Citrus Res & Educ Ctr, Lake Alfred, FL USA
[2] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL USA
[3] Univ Florida, Citrus Res & Educ Ctr, Dept Hort Sci, Lake Alfred, FL USA
关键词
ZINC-FINGER PROTEINS; GENE-EXPRESSION; HUANGLONGBING DISEASE; SWEET ORANGE; SALICYLIC-ACID; SINENSIS; FAMILY; RESISTANCE; FRUIT; RICE;
D O I
10.1371/journal.pone.0073742
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Huanglongbing (HLB) is the most destructive disease that affects citrus worldwide. The disease has been associated with Candidatus Liberibacter. HLB diseased citrus plants develop a multitude of symptoms including zinc and copper deficiencies, blotchy mottle, corky veins, stunting, and twig dieback. Ca. L. asiaticus infection also seriously affects the roots. Previous study focused on gene expression of leaves and fruit to Ca. L. asiaticus infection. In this study, we compared the gene expression levels of stems and roots of healthy plants with those in Ca. L. asiaticus infected plants using microarrays. Affymetrix microarray analysis showed a total of 988 genes were significantly altered in expression, of which 885 were in the stems, and 111 in the roots. Of these, 551 and 56 were up-regulated, while 334 and 55 were down-regulated in the stem and root samples of HLB diseased trees compared to healthy plants, respectively. Dramatic differences in the transcriptional responses were observed between citrus stems and roots to Ca. L. asiaticus infection, with only 8 genes affected in both the roots and stems. The affected genes are involved in diverse cellular functions, including carbohydrate metabolism, cell wall biogenesis, biotic and abiotic stress responses, signaling and transcriptional factors, transportation, cell organization, protein modification and degradation, development, hormone signaling, metal handling, and redox. Microscopy analysis showed the depletion of starch in the roots of the infected plants but not in healthy plants. Collapse and thickening of cell walls were observed in HLB affected roots, but not as severe as in the stems. This study provides insight into the host response of the stems and roots to Ca. L. asiaticus infection.
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页数:17
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