Growth Kinetics of Kyasanur Forest Disease Virus in Mammalian Cell Lines and Development of Plaque Reduction Neutralization Test

被引：0

作者：

Sarkale, Prasad ^{[1
]}

Shrivastava, Anish ^{[1
]}

Mohandas, Sreelekshmy ^{[1
]}

Patil, Savita Laxman ^{[1
]}

Kore, Pravin ^{[1
]}

Soman, Vinod ^{[1
]}

Yadav, Pragya D. ^{[1
]}

机构：

[1] ICMR Natl Inst Virol, 20-A,Dr Ambedkar Rd, Pune 411001, Maharashtra, India

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2019年 / 19卷 / 08期

关键词：

Kyasanur forest disease virus; mammalian cell lines; BHK-21; plaque reduction neutralization test; growth kinetics; INDIA;

D O I：

10.1089/vbz.2018.2405

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Kyasanur forest disease virus (KFDV) is a tick-borne flavivirus identified in 1957 in the Karnataka state of India causing fatalities in monkeys and humans. Even after the introduction of a vaccine in the endemic areas, hundreds of cases are reported every year. Being a high-risk category pathogen, the studies on this virus in India were limited till the past decade. The growth characteristics of this virus in various mammalian cell lines have not yet been studied. In this study, we have demonstrated the growth pattern of virus in BHK-21, Vero E6, Vero CCL81, rhabdomyosarcoma, porcine stable kidney, and Pipistrellus ceylonicus bat embryo cell lines, and found BHK-21 to be the best. We have developed KFDV plaque reduction neutralization test for the first time.

引用

页码：630 / 636

页数：7

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