RNA-seq analysis of synovial fibroblasts brings new insights into rheumatoid arthritis

被引:38
作者
Heruth, Daniel P. [1 ]
Gibson, Margaret [1 ]
Grigoryev, Dmitry N. [1 ]
Zhang, Li Qin [1 ]
Ye, Shui Qing [1 ,2 ]
机构
[1] Univ Missouri, Sch Med, Childrens Mercy Hosp & Clin, Dept Pediat, Kansas City, MO 64108 USA
[2] Univ Missouri, Sch Med, Childrens Mercy Hosp & Clin, Dept Biomed & Hlth Informat, Kansas City, MO 64108 USA
关键词
RNA-seq; Next generation sequencing; Rheumatoid arthritis; Synovial fibroblasts; Transcriptional regulation; GROWTH-FACTOR-BETA; GENE-EXPRESSION; CELLS; PATHWAY; GENOME; DIFFERENTIATION; OSTEOARTHRITIS; TRANSCRIPTOME; SIGNATURES; DISEASES;
D O I
10.1186/2045-3701-2-43
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Rheumatoid arthritis (RA) is a chronic autoimmune-disease of unknown origin that primarily affects the joints and ultimately leads to their destruction. Growing evidence suggests that synvovial fibroblasts play important roles in the initiation and the perpetuation of RA but underlying molecular mechanisms are not understood fully. In the present study, Illumina RNA sequencing was used to profile two human normal control and two rheumatoid arthritis synvovial fibroblasts (RASFs) transcriptomes to gain insights into the roles of synvovial fibroblasts in RA. Results: We found that besides known inflammatory and immune responses, other novel dysregulated networks and pathways such as Cell Morphology, Cell-To-Cell Signaling and Interaction, Cellular Movement, Cellular Growth and Proliferation, and Cellular Development, may all contribute to the pathogenesis of RA. Our study identified several new genes and isoforms not previously associated with rheumatoid arthritis. 122 genes were up-regulated and 155 genes were down-regulated by at least two-fold in RASFs compared to controls. Of note, 343 known isoforms and 561 novel isoforms were up-regulated and 262 known isoforms and 520 novel isoforms were down-regulated by at least two-fold. The magnitude of difference and the number of differentially expressed known and novel gene isoforms were not detected previously by DNA microarray. Conclusions: Since the activation and proliferation of RASFs has been implicated in the pathogenesis of rheumatoid arthritis, further in-depth follow-up analysis of the transcriptional regulation reported in this study may shed light on molecular pathogenic mechanisms underlying synovial fibroblasts in arthritis and provide new leads of potential therapeutic targets.
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页数:16
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