The mitochondria-targeted antioxidant MitoQ, attenuates exercise-induced mitochondrial DNA damage

被引:55
作者
Williamson, Josh [1 ]
Hughes, Ciara M. [2 ]
Cobley, James N. [3 ]
Davison, Gareth W. [1 ]
机构
[1] Ulster Univ, Sport & Exercise Res Inst, Newtownabbey, North Ireland
[2] Ulster Univ, Nursing & Hlth Res Inst, Newtownabbey, North Ireland
[3] Univ Highlands & Isl, Free Radical Res Grp, Ctr Hlth Sci, Inverness IV2 3JH, Scotland
关键词
Comet assay; Oxidative stress; Mitochondria; Exercise; ROS; DNA; INDUCED LIPID-PEROXIDATION; INDUCED OXIDATIVE STRESS; HYDROGEN-PEROXIDE; SKELETAL-MUSCLE; CELL-DEATH; FREE-RADICALS; NUCLEAR-DNA; LIFE-SPAN; VITAMIN-E; IN-VIVO;
D O I
10.1016/j.redox.2020.101673
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High-intensity exercise damages mitochondrial DNA (mtDNA) in skeletal muscle. Whether MitoQ a redox active mitochondrial targeted quinone can reduce exercise-induced mtDNA damage is unknown. In a double-blind, randomized, placebo-controlled design, twenty-four healthy male participants consisting of two groups (placebo; n = 12, MitoQ; n = 12) performed an exercise trial of 4 x 4-min bouts at 90-95% of heart rate max. Participants completed an acute (20 mg MitoQ or placebo 1-h pre-exercise) and chronic (21 days of supplementation) phase. Blood and skeletal muscle were sampled immediately preand post-exercise and analysed for nuclear and mtDNA damage, lipid hydroperoxides, lipid soluble antioxidants, and the ascorbyl free radical. Exercise significantly increased nuclear and mtDNA damage across lymphocytes and muscle (P < 0.05), which was accompanied with changes in lipid hydroperoxides, ascorbyl free radical, and a-tocopherol (P < 0.05). Acute MitoQ treatment failed to impact any biomarker likely due to insufficient initial bioavailability. However, chronic MitoQ treatment attenuated nuclear (P < 0.05) and mtDNA damage in lymphocytes and muscle tissue (P < 0.05). Our work is the first to show a protective effect of chronic MitoQ supplementation on the mitochondrial and nuclear genomes in lymphocytes and human muscle tissue following exercise, which is important for genome stability.
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页数:10
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