High-intensity exercise damages mitochondrial DNA (mtDNA) in skeletal muscle. Whether MitoQ a redox active mitochondrial targeted quinone can reduce exercise-induced mtDNA damage is unknown. In a double-blind, randomized, placebo-controlled design, twenty-four healthy male participants consisting of two groups (placebo; n = 12, MitoQ; n = 12) performed an exercise trial of 4 x 4-min bouts at 90-95% of heart rate max. Participants completed an acute (20 mg MitoQ or placebo 1-h pre-exercise) and chronic (21 days of supplementation) phase. Blood and skeletal muscle were sampled immediately preand post-exercise and analysed for nuclear and mtDNA damage, lipid hydroperoxides, lipid soluble antioxidants, and the ascorbyl free radical. Exercise significantly increased nuclear and mtDNA damage across lymphocytes and muscle (P < 0.05), which was accompanied with changes in lipid hydroperoxides, ascorbyl free radical, and a-tocopherol (P < 0.05). Acute MitoQ treatment failed to impact any biomarker likely due to insufficient initial bioavailability. However, chronic MitoQ treatment attenuated nuclear (P < 0.05) and mtDNA damage in lymphocytes and muscle tissue (P < 0.05). Our work is the first to show a protective effect of chronic MitoQ supplementation on the mitochondrial and nuclear genomes in lymphocytes and human muscle tissue following exercise, which is important for genome stability.
机构:
Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Lowes, Damon A.
;
Thottakam, Bensita M. V.
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Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Thottakam, Bensita M. V.
;
Webster, Nigel R.
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Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Webster, Nigel R.
;
Murphy, Michael P.
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MRC, Dunn Human Nutr Unit, Cambridge, EnglandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Murphy, Michael P.
;
Galley, Helen F.
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Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
机构:
Iowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Mao, Gaowei
;
Kraus, George A.
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Iowa State Univ, Dept Chem, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Kraus, George A.
;
Kim, Ikyon
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Iowa State Univ, Dept Chem, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Kim, Ikyon
;
Spurlock, Michael E.
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Iowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Spurlock, Michael E.
;
Bailey, Theodore B.
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Iowa State Univ, Dept Stat, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Bailey, Theodore B.
;
Zhang, Qijing
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h-index: 0
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Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Zhang, Qijing
;
Beitz, Donald C.
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机构:
Iowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
机构:
Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Lowes, Damon A.
;
Thottakam, Bensita M. V.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Thottakam, Bensita M. V.
;
Webster, Nigel R.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Webster, Nigel R.
;
Murphy, Michael P.
论文数: 0引用数: 0
h-index: 0
机构:
MRC, Dunn Human Nutr Unit, Cambridge, EnglandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
Murphy, Michael P.
;
Galley, Helen F.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, ScotlandInst Med Sci, Sch Med, Acad Unit Anaesthesia & Intens Care, Aberdeen AB25 2ZD, Scotland
机构:
Iowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Mao, Gaowei
;
Kraus, George A.
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Dept Chem, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Kraus, George A.
;
Kim, Ikyon
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Dept Chem, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Kim, Ikyon
;
Spurlock, Michael E.
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Spurlock, Michael E.
;
Bailey, Theodore B.
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Dept Stat, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Bailey, Theodore B.
;
Zhang, Qijing
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Zhang, Qijing
;
Beitz, Donald C.
论文数: 0引用数: 0
h-index: 0
机构:
Iowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA
Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USAIowa State Univ, Interdept Grad Program Nutr Sci, Ames, IA 50011 USA