Utility of two novel multiplexing assays for the detection of gastrointestinal pathogens - a first experience

被引：25

作者：

Kahlau, Pascal ^{[1
]}

Malecki, Monika ^{[1
]}

Schildgen, Verena ^{[1
]}

Schulz, Christine ^{[2
]}

Winterfeld, Ingo ^{[2
]}

Messler, Sabine ^{[2
]}

Mattner, Frauke ^{[2
]}

Schildgen, Oliver ^{[1
]}

机构：

[1] Univ Witten Herdecke, Klinikum Privaten, Kliniken Stadt Koln gGmbH, Inst Pathol, D-51109 Cologne, Germany

[2] Univ Witten Herdecke, Klinikum Privaten, Kliniken Stadt Koln gGmbH, Inst Hyg, D-51109 Cologne, Germany

来源：

SPRINGERPLUS | 2013年 / 2卷

关键词：

D O I：

10.1186/2193-1801-2-106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Cause for gastroenteritis range from viral, bacterial to parasitic pathogens. Rapid Multiplexing techniques like ProGastro_SSCS and xTAG_GPP can detect broad panels of pathogens simultaneously. We performed a field test with a total number of 347 stool samples from adult hospitalized patients that were tested with the Luminex xTAG GPP assay; of the 157 samples positively tested for at least one pathogen by xTAG GPP a total number of 30 samples was retested with the ProGastro SSCS assay. Assays were compared to standard routine diagnostics. Findings: Multiplexing significantly reduced the time to the initial identification of a pathogen. Moreover, multiplexing detected pathogens for which a diagnostic assays was not requested by the physician and thus may be an important tool for avoiding nosocomial outbreaks. Conclusion: This first frontline approach with these assays approves their utility compared to conventional microbiological methods.

引用

页码：1 / 6

页数：6

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