The protein CTCF is required for the enhancer blocking activity of vertebrate insulators

被引：874

作者：

Bell, AC ^{[1
]}

West, AG ^{[1
]}

Felsenfeld, G ^{[1
]}

机构：

[1] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

CELL | 1999年 / 98卷 / 03期

关键词：

D O I：

10.1016/S0092-8674(00)81967-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An insulator is a DNA sequence that can act as a barrier to the influences of neighboring cis-acting elements, preventing gene activation, for example, when located between an enhancer and a promoter. We have identified a 42 bp fragment of the chicken beta-globin insulator that is both necessary and sufficient for enhancer blocking activity in human cells. We show that this sequence is the binding site for CTCF, a previously identified eleven-zinc finger DNA-binding protein that is highly conserved in vertebrates. CTCF sites are present in all of the vertebrate enhancer-blocking elements we have examined. We suggest that directional enhancer blocking by CTCF is a conserved component of gene regulation in vertebrates.

引用

收藏

页码：387 / 396

页数：10

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