Individual variability of mytimycin gene expression in mussel

被引：20

作者：

Cantet, Franck ^{[1
]}

Toubiana, Mylene ^{[1
]}

Parisi, Maria-Giovanna ^{[2
]}

Sonthi, Molruedee ^{[1
]}

Cammarata, Matteo ^{[2
]}

Roch, Philippe ^{[1
]}

机构：

[1] Univ Montpellier 2, Ecol Syst Marins & Cotiers EcoSym UMR5119, CNRS, F-34095 Montpellier 05, France

[2] Univ Palermo, Marine Immunobiol Lab, I-90123 Palermo, Italy

来源：

FISH & SHELLFISH IMMUNOLOGY | 2012年 / 33卷 / 03期

关键词：

Antifungal; Antimicrobial; Gene expression; Challenge; Innate immunity; MYTILUS-GALLOPROVINCIALIS; ANTIMICROBIAL PEPTIDE; HEAT-SHOCK; HEMOCYTES; EDULIS; SUBPOPULATIONS; POLYMORPHISM; INVOLVEMENT; BACTERIA; MYTICIN;

D O I：

10.1016/j.fsi.2012.06.020

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The antifungal peptide mytimycin (MytM) is synthesized by hemocytes of the Mediterranean mussel, Mytilus galloprovincialis. In addition to sequence and gene structure diversities previously reported from pooled hemocytes, the present report focused on the expression of mytm gene in individual M. galloprovincialis, before and after challenge. Within untreated mussel. MytM mRNA was observed by ISH in about 42% of circulating hemocytes, characterized by large, diffuse nucleus. Injection with Fusarium oxysporum increased such percentage, but in only some of the mussels. Similarly, MytM gene expression increased after injection in only some of the mussels, as measured by qPCR. Responders and not responders are common evidence in any given population of organisms. Nevertheless, even if the use of proper pool size selection has been practised to find out and evaluate the most common response trends, individual analyses must be regarded as optimal. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：641 / 644

页数：4

共 25 条

[1] MORPHOFUNCTIONAL STUDY OF THE HEMOCYTES OF THE BIVALVE MOLLUSK MYTILUS-GALLOPROVINCIALIS WITH EMPHASIS ON THE ENDOLYSOSOMAL COMPARTMENT [J].